肺癌细胞在缺氧状态下体外侵润力的变化及分子基础  

Effect of Hypoxia on in Vitro Invasion of Lung Carcinoma Cells and Its Molecular Basis

作　　者：胡振红[1] 彭琪琳[1] 汪君[1] 傅祖红[1] 司斌[1] 刘志遐[1] 

机构地区：[1]广州军区武汉总医院呼吸内科,湖北武汉430070

出　　处：《中国医师杂志》2004年第2期163-165,共3页Journal of Chinese Physician

摘　　要：目的　探讨缺氧对肺癌细胞体外侵润力的影响及其分子基础。方法　将小细胞肺癌细胞H12 8分别置于常氧、低氧和无氧环境中培养 48h后 ,用羊膜内皮细胞模型测定各组细胞的侵润能力。同时测定并分析了上皮钙粘附素、β1 -整合素的表达量。结果　与常氧组相比 ,低氧组细胞侵润能力显著高于常氧组。低氧组E -钙粘附素表达显著降低及 β1 -整合素表达量显著增强 ,无氧组两者均显著降低。结论　适度缺氧可以下调肺癌细胞表达E -钙粘附素 ,增加 β1 -整合素表达 ,增强癌细胞侵润力。Objective To explore the effect of hypoxia on the in vitro invasion of lung carcinoma cells and its molecular basis. Methods Small cell lung carcinoma cells(H128) were exposed to hypoxia(5%O 2,5%CO 2,90%N 2) or anoxia(95%N 2,5%CO 2)condition for 48 hours. The invasive ability of the cancer cells was assayed with HABM-HEM model. The expression of E-cadherin and β 1-integrin on the cells was also assayed by flow cytometry. Results Compared with normoxia group, the invasive ability of the cancer cells increased, the expression of E-cadherin decreased, and the expression of β 1-integrin increased in hypoxia group. In anoxia group, the invasive ability and the expression of E-cadherin and β 1-integrin all decreased compared with normoxia group. Conclusions Moderate hypoxia can down-regulate the expression of E-cadherin, up-regulate the expression of β 1-integrin, and increase the invasion of carcinoma cells. But serious hypoxia can decrease the expression of adhesive molecules and the invasion of the cancer cells.

关 键 词：肺癌 缺氧状态 分子机制 癌细胞侵润力 细胞粘附分子 

分 类 号：R734.2[医药卫生—肿瘤]