胃癌及癌前期端粒酶活性与病变基因表达在细胞凋亡中的相互关系及临床意义  

Investigation of clinical significance of telomerase and lesion genetic expression and their correlation in apoptosis of gastric carcinoma and precancerous lesion cell

作　　者：潘华君[1] 俞晓宇[1] 宫玲玲[1] 王枫[1] 

机构地区：[1]江苏省无锡市第五人民医院,214073

出　　处：《浙江临床医学》2004年第2期83-84,共2页Zhejiang Clinical Medical Journal

摘　　要：目的 探讨胃癌及癌前期端粒酶与病变基因表达在细胞凋亡中的相互关系及临床意义。方法 分别用 (1)端粒酶重复扩增法对82例胃粘膜病变组织标本端粒酶RNA活性检测 ;(2)用免疫组化染色法对79例胃粘膜病变组织标本检测 p53、bcl-2蛋白的表达。结果 端粒酶活性检测组中GC阳性率为100 %,DYS为58.33%,两者具有非常显著性统计学差异 (p<0.01) ;免疫组化组中GC :p53与bcl-2比较有显著性差异 ( p<0.05)。DYS组 :p53与bcl-2比较有极显著性统计学差异 ( p<0.01)。结论 通过用二种不同的方法分别检测端粒酶活性及病变基因p53、bcl-2的表达 ,来分析研究两者在细胞凋亡中的相互关系 ,探讨其临床意义 ,是一项有意义的研究。从分析中可以看出 ,不论是端粒酶或p53、bcl-2 ,它们在病变组织中的表达必然的内在联系 ,为今后在探索肿瘤的发病机制上提示出 :(1)随着肿瘤的生长 ,端粒酶和bcl-2活性将增加 ,而p53将有条件的变化 ;(2)在研究端粒酶时 ,把端粒定量更具临床意义 ;(3) p53、bcl-2高表达在细胞凋亡过程中发生在端粒酶激活之前 。Objective To explore clinical significance of telomerase and lesion genetic expression and their correlation in apoptosis of gastric carcinoma and precancerous lesion cell. Methods 1) Telomerase RNA activities of gastric mucosa lesion specimen in 82 cases were detected by telomerase duplication amplification.2) The expressions of p53 and bcl-2 gene of specimen in 79 cases were detected by immunohistochemistry staining. Results The positive ratios of telomerase in GC and DYS group were 100%and 58.33%respectively. There was significant difference between them. The expression ratios of p53 gene and bcl-2 gene were significantly different both in GC and DYS groups.Conclusion The expressions of telomerase, p53 and bcl-2 in gastric lesion correlated with each other. The results suggested that the following question should be explored: 1) The activity of telomerase and bcl-2 genetic expression increase, but genetic expression p53 changes conditionally with the development of tumor. 2) Should Telomere be quantified before telomerase detection? 3) Do p53 and bcl-2 gene express before the activation of telomerase in cell apoptosis

关 键 词：胃癌 癌前期 端粒酶活性 基因表达 细胞凋亡 胃粘膜 

分 类 号：R735.2[医药卫生—肿瘤]