180例急性白血病流式细胞术免疫分型的特点分析  

Characteristic Analysis of Actue Leukemia Immunophenotyping in 180 Cases by Flow Cytometry

作　　者：姚志娟[1] 廖丽[1] 党永辉[2] 张婧[1] 田瑛[1] 冯凯[1] 齐聪燕[1] 王谨峰[1] 陈虎[1] 

机构地区：[1]军事医学科学院307医院,北京100039 [2]深圳市龙岗区人民医院,深圳518172

出　　处：《中国实验血液学杂志》2004年第1期83-85,共3页Journal of Experimental Hematology

摘　　要：为了探讨急性白血病的免疫表型特征与诊断价值 ,采用流式细胞术三色免疫荧光直接标记技术检测白血病患者 ,根据抗体积分系统进行分型。结果显示 ,三染色标记法检测 180例白血病患者中 ,急性淋巴细胞白血病(ALL)患者除均表达淋系抗原外 ,同时有 4 9.4 %伴髓系抗原 (CD13,CD33)的表达 ;急性髓细胞白血病 (AML)患者除均表达髓系抗原外 ,同时有 4 3.2 %伴淋系抗原 (CD4 ,CD7)表达。结论 :流式细胞仪三色免疫荧光直接标记法进行白血病免疫分型能确诊一些特殊类型的白血病 ,对于急性白血病的诊断治疗和预后判断均有很大帮助。To study the actue Leukemia immunophenotype and its diagnosis value, three color direct immunofluorescece staining methods of flow cytometry and immunophenotype of antibody integration system were used for detection of 180 cases of acute leukemia. The results showed that patients with ALL expressed lymphocyte antigen, and 49.4% patients with ALL accompanied myeloid antigen; all patients with AML expressed myeloid antigen, and 43.2% patients with AML accompained lymphocyte antigen. In conclusion, leukemia immunophenotyping by three-color direct immunofluore staining methods could define some particular types of leukemia with an important value in diagnosis, treatment and predicting prognosis of acute leukemia.

关 键 词：流式细胞术 免疫分型 急性白血病 

分 类 号：R733.71[医药卫生—肿瘤]