乙醇脱氢酶3的遗传多态性对摄入乙醇唾液和全血中乙醛含量的影响  

Effect of genetic polymorphism of alcohol dehydrogenase 3 on acetaldehyde concentration in saliva and blood following ethanol ingestion

作　　者：李俊杰[1] 

机构地区：[1]暨南大学医学院第一附属医院临床药理研究室,广东广州510630

出　　处：《中国药理学与毒理学杂志》2004年第1期53-56,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：广东省卫生厅医学科学技术研究项目(WSTJJ 2 0 0 2 12 12 43 0 10 51970 0 912 3 0 40 );教育部留学回国人员科研启动基金资助项目 (2 0 0 3 40 6)~~

摘　　要：目的　探讨不同乙醇脱氢酶 3(ADH3)基因型对乙醇体内氧化的影响。方法　采用PCR扩增反应测定ADH3基因型 ,将 2 2名健康受试者分为ADH31 -1 ,ADH31 -2 和ADH32 -2 3组。受试者一次po 0 .3g·kg-1 乙醇后 ,采用高效液相色谱荧光法测定 4h内唾液和全血中的乙醛浓度经时过程。结果ADH31 -1 组的乙醛唾液cmax为 (1 2 .5± 2 .3) μmol·L-1 ,AUC为 (1 .4± 0 .4)mmol·min·L-1 ;ADH31 -2 组唾液cmax为 (9.4±1 .7) μmol·L-1 ,AUC为 (1 .1±0 .3)mmol·min·L-1 ;ADH32 -2 组唾液cmax为 (8.7± 2 .2 )μmol·L-1 ,AUC为 (0 .93± 0 .1 9)mmol·min·L-1 。ADH31 -1 组的唾液中乙醛浓度显著高于ADH31 -2 和ADH32 -2 组 ,但全血中的组间差异不如唾液中明显。结论　基因型为ADH31 -1 的个体在摄入乙醇后唾液中乙醛量显著增加 ,提示ADH31 -1AIM To explore the effect of different genotype of alcohol dehydrogenase 3(ADH3) on the oxidation of ethanol in vivo. METHODS Twenty two volunteers were divided into ADH3 1-1 , ADH3 1-2 , ADH3 2-2 groups using PCR amplification. Volunteers were given an oral acute dose of 0.3 g ethanol·kg -1 . Salivary and blood acetaldehyde concentrations were measured using HPLC with fluorescence detection over a period of 4 h. RESULTS In ADH3 1-1 group salivary c max and AUC were (12.5±2.3) μmol·L -1 , (1.4±0.4) min·mmol·L -1 ; In ADH3 1-2 group salivary c max and AUC were (9.4±1.7)μmol·L -1 , (1.1± 0.3)min ·mmol·L -1 ;In ADH3 2-2 group salivary c max and AUC were (8.7±2.2) μmol·L -1 , (0.93 ±0.19) min·mmol·L -1 . There was not so significant difference in acetaldehyde content in blood as in salivary among the three groups.CONCLUSION Salivary acetaldehyde concentrations are significantly increased in individuals with ADH3 1-1 , it may implicate that the risk of pathological impairment induced by acetaldehyde is enhanced in individuals with this genotype.

关 键 词：乙醇脱氢酶3 遗传多态性 唾液 血液 乙醛含量 

分 类 号：R363[医药卫生—病理学]