淫羊藿总黄酮与雌激素合用对去卵巢大鼠骨质疏松的影响  

The effects of epimedium pubescens flavonoids combined with diethylstilbestrol on ovariectomy induced osteopenia in rats

作　　者：许碧连[1] 吴铁[1] 崔燎[1] 刘钰瑜[1] 

机构地区：[1]广东医学院药理学教研室,湛江524023

出　　处：《中国药理学通报》2004年第2期218-222,共5页Chinese Pharmacological Bulletin

基　　金：广东医学院青年基金资助课题 ;NoXQ0 2 10

摘　　要：目的　研究淫羊藿总黄酮 (EF)与己烯雌酚 (DES)合用对去卵巢大鼠骨质疏松的影响。方法　大鼠双侧卵巢去除术后预防用药 90d ,用骨组织形态计量学等方法 ,测定大鼠胫骨近端松质骨静态参数和动态参数 ,并观察血清生化指标和子宫内膜厚度。结果　DES 2 2 5 μg·kg-1·d-1可对抗去卵巢大鼠体重增加、血清总胆固醇增加、骨转换增高和骨丢失 ,但是使子宫内膜厚度增加。单用EF 30 0mg·kg-1·d-1对骨丢失没有对抗作用 ,DES 2 2 5 μg·kg-1·d-1和EF30 0mg·kg-1·d-1联合用药效应超过DES单独应用 ,而且不刺激子宫内膜增加。结论　EF与DES联合用药对去卵巢大鼠骨丢失的骨量增加有协同作用 ,而且没有刺激子宫的副作用。AIM To determine whether epimedium pubescens flavonoids(EF) in combination with diethylstilbestrol(DES) can completely prevent bone loss in ovariectomized rats. METHODS Sixty Sprague-Dawley female rats at age of 4 months were divided into six groups. Ten rats were sacrificed at age of 4.5 months before operation. The other rats were sham-operated and treated orally with vehicle or ovariectomized (OVX) and treated orally with either vehicle, or diethylstilbestrol(DES) at 22.5 μg·kg -1 ·d -1 , or EF at 300 mg·kg -1 ·d -1 , or EF 300 mg·kg -1 ·d -1 in combination with DES 22.5 μg·kg -1 ·d -1 for 90 days. Double in vivo fluorochrome labeling was administrated. The undecalcified longitudinal proximal tibial metaphyseal sections were used for [FQ(9*2。46,X-WZ]-the bone histomorphometric analysis. RESULTS DES prevented OVX-induced bone loss and decreased body weight gain and total serum cholesterol,but it increased uterine luminal epithelial thickness.Combination of EF with DES completely prevented OVX-induced bone loss and did not increase uterine luminal epithelial thickness.The effect of the combination in preventing bone loss was more significant than that of DES. CONCLUSION EF in combination with DES showed synergistic effect on prevention of osteoporosis induced by ovariectomy.-

关 键 词：淫羊藿总黄酮 己烯雌酚 去卵巢大鼠 骨组织形态计量学 骨质疏松 

分 类 号：R-332[医药卫生] R284.1