三七总甙对NB4细胞促凝活性及诱导分化的影响  

Effect of Panax Notoginseng Saponin on Procoagulant Activity and Differentiation Induction in NB4 Cells

作　　者：李晓红[1] 董作仁[2] 郝洪岭[2] 刘泽林[2] 罗建民[2] 姚丽[2] 杨敬慈[2] 

机构地区：[1]河北省中医院 [2]河北医科大学第二医院血液科

出　　处：《中国中西医结合杂志》2004年第1期63-66,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：河北省卫生厅中医药;中西医结合科学研究计划课题

摘　　要：目的 :研究三七总甙对NB4细胞促凝活性和组织因子表达及诱导分化作用的影响。方法 :用三七总甙处理NB4细胞 ,测定复钙时间观察NB4细胞的促凝活性 (procoagulantactivity ,PCA) ,RT PCR检测TF mRNA的表达 ;采用四唑蓝 (MTT)法检测三七总甙对NB4细胞增殖抑制作用 ;以NBT还原实验、细胞形态学观察及流式细胞术分析研究三七总甙对NB4细胞诱导分化的影响。结果 :(1)不同浓度三七总甙均可显著延长NB4细胞的复钙时间 ,降低NB4细胞的PCA水平 ,呈时间浓度依赖性 ,同时下调组织因子TF mRNA的表达 ;(2 )三七总甙可部分抑制NB4细胞的增殖 ;(3)三七总甙可提高NB4细胞NBT还原能力(P <0 0 5 ) ,细胞形态方面显示单核 /巨噬细胞分化趋势。结论 :三七总甙可降低NB4细胞的促凝活性及TF mRNA的表达 ,诱导NB4细胞部分分化 ,有望作为新的抗凝药物。Objective:To investigate the effect of Panax notoginseng saponin (PNS) on procoagulant activity (PCA) and differentiation induction in NB4 cells. Methods:After NB4 cells were treated with PNS, the recalcification time, PCA and TF-mRNA expression in NB4 cells were tested by RT-PCR. The inhibitory effect of PNS on NB4 cell proliferation was analysed by MTT method, NBT assay, cell morphological observation and flow cytometry. Results:(1) PNS of all concentrations could significantly prolong the recalcification time and lower the PCA level in NB4 cells in time-concentration-dependent manner. Simultaneously it down-regulated the expression of TF-mRNA. (2) PNS could partially inhibit the NB4 cell proliferation. (3) PNS could raise the NBT reducing capabitity of NB4 cells (P<0.05). And morphological examination showed the differentiating tendency of monocyte and macrophage. Conclusion:PNS could reduce the procoagulant activity and TF-mRNA expression in NB4 cells, and partially induce the differentiation of NB4 cells, therefore, it is hopeful to be a new anti-coagulant agent.

关 键 词：三七总甙 NB4细胞 促凝活性 诱导 分化 组织因子 细胞分化 

分 类 号：R733.7[医药卫生—肿瘤]