二温式RT-PCR检测对虾Taura综合征病毒的研究  

Two-temperature reverse transcription polymerase chain reaction (RT-PCR) for the detection of taura syndrome virus in Penaeus vannamei

作　　者：庞耀珊[1] 谢芝勋[1] 何竞铭[1] 邓显文[1] 莫友群[2] 

机构地区：[1]广西兽医研究所,广西南宁530001 [2]广西海洋研究所,广西北海536000

出　　处：《海洋科学》2004年第2期54-57,共4页Marine Sciences

基　　金：广西壮族自治区科技攻关项目;水产畜牧局科研基金资助项目

摘　　要：设计了一对能扩增大小为231bp对虾Taura综合征病毒 (TSV)某段基因的特异性引物 ,优化建立了能快速检测TSV的二温式RT-PCR。特异性和敏感性试验结果表明 ,二温式RT-PCR能对3个试验用TSV毒株的RNA进行扩增 ,并得到与预期大小一致的231bp的扩增产物 ,而其它3种对虾病原则无相同大小的特异性扩增产物出现 ;RT-PCR最低能检测到1pg的TSVRNA。应用RT-PCR对320份分别来自广西沿海不同对虾养殖场的对虾样品进行检测 ,结果一共有85份检出TSV。这说明了TSV在广西沿海地区养殖对虾中已呈现出区域性流行趋势 ,同时也显示了RT-PCR在TSV临床检测中具有较高的实用性。A two-temperature RT- PCR was developed and optimized for the detection of Taura syndrome virus (TSV). Primers originated from the gene sequence of TSV. A TSV specific 231-base pair (bp) cDNA product was amplified using primers from 3 field strains of TSV not from 3 other shrimp pathogenic virus and bacteria.Using the RT-PCR technique, TSV was detected in 85 of 320 clinical cultivated penaeid shrimps in Guangxi. Results demonstrated that this two-temperature RT-PCR could be used as a rapid and sensitive clinical detection method for the diagnosis of TSV.

关 键 词：南美白对虾 TAURA综合征病毒 二温式 RT—PCR 检测 

分 类 号：S941.41[农业科学—水产养殖]