氟诱导牙胚分泌期造釉细胞凋亡的实验研究  

The effect of fluoride on the secretory ameloblast apoptosis

作　　者：邓转云[1] 陈明霞[2] 侯铁舟[1] 袁宏民[1] 

机构地区：[1]西安交通大学口腔医学院口腔内科,陕西西安710004 [2]西安交通大学医学院电子显微镜室,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2004年第1期39-41,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的　探讨氟与分泌期造釉细胞凋亡的关系 ,进一步明确氟斑牙的发病机制。方法　通过建立孕鼠急性氟中毒动物模型 ,用透射电镜、缺口末端标记法 (TUNEL)观察胎鼠牙胚分泌期造釉细胞凋亡。结果　孕鼠高氟引起胎鼠牙胚分泌期造釉细胞下囊腔的形成 ,囊腔上方的造釉细胞发生了程序性细胞凋亡 (PCD)。Objective To elucidate the relationship between f luoride and cell apoptosis of ameloblasts and to further study the mechanism of dental fluorosis. Methods By establishing an experimental m odel of fluoride intoxication in pregnant C57BL/6N, we observed apoptosis in the fetal secretory ameloblasts in mice by means of transmission electron microscop e and TUNEL. Results High fluoride exposure in pregnant mic e could cause subameloblastic cysts in first lower molar of fetal mice. Programm ed cell death (PCD) occurred in the disturbed ameloblasts above cysts. Conclusion Intoxicat ion mechanism of fluoride on ameloblasts may occur by means of PCD.

关 键 词：氟 牙胚 造釉细胞 细胞凋亡 实验 毒性作用 

分 类 号：R781.2[医药卫生—口腔医学] R329.2[医药卫生—临床医学]