螺内酯对实验大鼠肝组织TGFβ1、PDGF-BB及α-SMA表达的影响  

The effect of spironolactone on the expression of TGF-β_1 , PDGF-BB and α-SMA in hepatic fibrosis tissues in rats

作　　者：贾皑[1] 苌新明[1] 张盈涛[1] 厉英超[1] 常英[1] 

机构地区：[1]西安交通大学第一医院消化科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2004年第1期64-66,75,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的　探讨螺内酯预防肝纤维化的作用机制。方法　SD大鼠 90只 ,随机分为 3组。正常对照组 (8只 ) :正常饮食 ,皮下注射花生油 ;模型组 (42只 ) :复合因素制成肝纤维化模型 ;螺内酯预防组 (40只 ) :造模方法同模型组 ,螺内酯 10 0mg·kg- 1·d- 1灌胃。分别于第 2周、4周、6周、8周末随机处死 8只大鼠 ,取肝组织用免疫组化法检测各组肝组织内转化生长因子 β1(TGFβ1)、血小板衍生生长因子 (PDGF BB)及α 平滑肌动蛋白 (α SMA)含量。结果　螺内酯预防组TGFβ1、PDGF BB及α SMA在肝组织中的表达均较模型组明显减少 (P <0 .0 5或P <0 .0 1)。 结论　螺内酯可通过降低TGFβ1、PDGFObjective To investigate the preventive effect of spironolactone on hepatic fibrosis. Methods Ninety SD rats were randomly divided into three groups. Control group consisted of 8 rats that , fed by normal food, were injected with peanut oil subcutaneously. Model group consisted of 42 rats whose liver fibrosis was induced by compound factors. Spiro nolactone-prevention group consisted of 40 rats that were given 100 mg·kg -1 spironolactone per day, by the same methods of making models as those of the model group. At the end of weeks 2, 4, 6 and 8, 8 rats were randomly taken out of model group and spironol actone group and then were sacrificed. The expressions of TGF- β 1 , PDGF- BB and α -SMA in hepatic tissues were detected with immunohistochemical met hods. Results The expressions of TGF- β 1 , PDGF-BB a nd α -SMA in spironolactone group decreased greatly than those in model gro up ( P <0.05 or P <0.01). Conclusion Spironolactone may prevent the formation of hepa tic fibrosis by decreasing the secretion of TGF- β 1 and PDGF-BB and inhi biting the activation of hepatic stellate cell .

关 键 词：螺内酯 实验 大鼠 肝组织 TGFΒ1 PDGF-BB α-SMA 肝纤维化 转化生长因子β1 血小板衍生生长因子 Α-平滑肌动蛋白 肝星状细胞 

分 类 号：R575.2[医药卫生—消化系统] R977.6[医药卫生—内科学]