结直肠癌组织中胸苷酸磷酸化酶的表达及其临床意义  

Expression of Thymidine Phosphorylase in Human Colorectal Carcinoma and Its Clinical Significance

作　　者：肖菊香[1] 孙红[1] 杨谨[1] 李春丽[1] 韩梅[1] 李蓉[1] 

机构地区：[1]西安交通大学第一医院肿瘤内科,陕西西安710061

出　　处：《癌症》2004年第3期303-305,共3页Chinese Journal of Cancer

摘　　要：背景与目的:血管生成已被公认是与肿瘤的生长和侵袭有关,近年研究表明胸苷酸磷酸化酶(thymidinephosphorylase,TP)不仅参与肿瘤新生血管形成,而且可增强5-氟尿嘧啶前药的化疗敏感性,但其在结直肠癌中的作用以及对预后的影响尚未得出一致的结论。本研究检测TP在人结直肠癌组织中的表达水平,并探讨其临床意义。方法:以西安交通大学第一医院行根治术且随访满5年的70例结直肠癌患者的库存组织标本为材料,采用免疫组织化学方法检测其TP表达水平。结果:TP表达主要见于肿瘤细胞胞浆之中,TP阳性表达患者40例,占57.14%。TP在不同Dukes分期和不同病理组织学分级组织中的表达水平具有显著性差异(P=0.007和P=0.002)。Kaplan-Meier生存率分析提示,TP阳性表达者5年生存率为33.33%,而TP阴性表达者则为73.33%(P=0.000),结果提示TP阳性表达患者的预后较阴性表达者差。结论:TP表达可作为判断结直肠癌患者预后的参考指标之一。BACKGROUND & OBJECTIVE: Angiogenesis has been well known to be a factor related to the tumor growth and metastasis. Recent studies have reported that thymidine phosphorylase (TP) is identical with platelet-derived endotheli al cell growth factor. It not only plays a role in the induction of the tumor an giogenesis, but also enhances the chemotherapeutical sensitivity of the tumor ce lls to prodrug of 5-fluorouracil. However, until now, the complete identity of view about the function of TP expression in colorectal carcinoma and its prognos tic significance has not been reached. The present study was to explore the TP e xpression level in human colorectal carcinoma and its clinical significance. MET HODS: Seventy archived samples of the patients with colorectal carcinoma were de tected by immunohistochemical staining. These patients had undergone radical sur gical treatment at the First Hospital of Xian Jiaotong University and had been followed up for 5 years after operation. RESULTS: TP expression was predominant ly observed in the cytoplasm of the tumor cells. The positive rate was 57.14%(4 0/70). TP expression levels in the patients with different Dukes, stages and his topathological grades showed obvious difference (P=0.007 and 0.002,respectively) . By Kaplan-Meier methods, the survival of TP positive expression group was sho rter than that of TP negative expression group, and the five-year survival rate s of TP positive expression group and TP negative expression group were 33.33% and 73.33%(P=0.000). The results indicated that the prognosis of the patients with TP positive expression was poorer than that of patients with TP negative e xpression. CONCLUSION: These findings suggest that TP expression might be one of the important prognostic indicators for colorectal carcinoma patients, and TP d etection in colorectal carcinoma patients might served as a parameter for design ing therapeutic schedule and choosing prodrug of 5-fluorouracil.

关 键 词：结直肠癌 癌组织 胸苷酸磷酸化酶 基因表达 肿瘤 

分 类 号：R735.3[医药卫生—肿瘤]