枯否细胞条件培养基及混合血清对肝星状细胞HSC-T6的刺激作用  

Activation of hepatic stellate cell strain HSC-T6 by Kupffer cell-conditioned medium and mixed sera

作　　者：吴强[1] 杨雁[2] 张宵翔[2] 薛绍礼[3] 邹宇宏[2] 陈敏珠[2] 

机构地区：[1]安徽医科大学病理学教研室,合肥230032 [2]安徽医科大学临床药理研究所,合肥230032 [3]安徽医科大学细胞工程学教研室,合肥230032

出　　处：《安徽医科大学学报》2004年第1期9-12,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学研究基金 (编号 :990 44 12 6)

摘　　要：目的　探讨枯否细胞条件培养基 (KCCM )、混合的新生牛血清 (NBS)和大鼠血清 (RS)对肝星状细胞 (HSC)增殖和胶原合成的促进作用。方法　采用KCCM、单纯NBS及混合血清刺激大鼠HSC T6细胞 ,用3 H TdR和3 H 脯氨酸掺入法检测HSC增殖活性和胶原合成状况。结果　不同刺激剂作用 4 8h后 ,HSC均体积增大 ,胞突增多延长 ,核分裂像增多。 4 8h的3 H TdR和 96h的3 H 脯氨酸掺入明显增加。结论　混合血清对HSC T6的综合激活作用较KCCM和单纯 10 %NBS的作用明显 ,能明显促进其增殖和胶原合成 。Objective To study the activation of hepatic stellate cells (HSC) by Kupffer cell-conditioned medium (KCCM), new bovine serum (NBS) and rat serum. Methods Rat HSC-T6 cells were cultured in DMEM with or without KCCM, NBS and normal rat serum. HSC proliferation and collagen secretion were measured with 3H-TdR and 3H-proline incorporation. HSC morphology was observed under light microscope. Results After culturing with KCCM and sera, HSCs enlarged with protrudes elongating and increasing, and mitotic figures increased. 3H-TdR uptake at 48 h and 3H-proline uptake at 96 h were both increased remarkably in a dose-dependent manner. Conclusion Ten percent of new bovine serum together with normal rat serum and KCCM may activate HSC-T6 effectively, accelerating both its DNA and collagen syntheses, which may serve as a model for the study of hepatic fibrosis.

关 键 词：枯否细胞 混合血清 肝星状细胞 HSC-T6 刺激作用 条件性培养基 肝纤维化 

分 类 号：R575.2[医药卫生—消化系统]