脑出血灶周缺氧诱导因子-1α、热休克蛋白70的表达  

Expression of HIF-1α and HSP70 in Perihematoma with Intracerebral Hemorrhage

作　　者：唐洲平[1] 郭守刚[1] 彭岚[1] 朱遂强[1] 方思羽[1] 张苏明[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《华中科技大学学报（医学版）》2004年第1期49-51,108,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：卫生部临床学科重点项目基金资助(No.97070241)

摘　　要：目的 探讨缺氧诱导因子-1α(HIF-1α)、热休克蛋白70(HSP70)在脑出血灶周脑组织中的表达及其意义。方法 应用免疫组织化学技术检测37例脑出血灶周脑组织中HIF-1α、HSP70的表达。结果 37例脑出血灶周脑组织中HIF-1α阳性率为40.5％,其中出血量>60 ml者HIF-1α阳性率为88.9％,与其他出血量者(30-45 ml,46-60 ml)比较差异有显著性意义(P<0.05)。HIF-1α表达与血肿作用时间明显相关,随着出血后手术时间的推迟而增加(P<0.05)。HIF-1与HSP70的表达呈正相关(n=15,r=0.82,P<0.01)。结论 脑出血后HIF-1α的表达与出血量及血肿作用时间相关,为判断脑出血灶周缺血半暗带的存在提供了依据;HSP70有神经细胞保护及抑制凋亡的作用。Objective To evaluate the expression of hypoxia inducible factor-1α (HIF-1α) and heat shock protein 70 (HSP70) in perihematoma with intracerebral hemorrhage, and to explore their possible roles after cerebral hemorrhage. Methods The expression levels of HIF-1α and HSP70 were assessed by inmunohistochemistry straining. Results In 37 cases of hypertensive hemorrhage, positive rate for HIF-la was 40. 5 %. The positive rate in the patients with the volume of blood loss >60 ml (88. 9 %) was significantly higher than those with the volume of blood loss being 30-45 ml or 46-60 ml. The HIF-1α expression was closely related with the choice of the operating opportunity and increased with the delay of the operating time (P<0. 05). There were a positive correlation between HIF-1α labeling index and HSP70 index (n=15, r=0. 82, P<0. 01). Conclusion The expression of HIF-1αwas closely related with the choice of operating opportunity and the volume of blood loss, which provided a foundation that the ische-mic penumbra existed in the perihematoma. HSP70 could protect nerve cells and inhibit apoptosis.

关 键 词：脑出血 缺氧诱导因子-1Α 热休克蛋白70 表达 

分 类 号：R743.34[医药卫生—神经病学与精神病学]