水蛭桃仁汤对肝纤维化大鼠HSC活化及TGFβ1表达的影响  

Effects of Decoction of Leech and Peach Kernel on Activation of HSC and Expression of TGF-β1 in Rat Liver Fibrosis

作　　者：晏丹[1] 陈建明[2] 舒赛男[3] 

机构地区：[1]武汉科技大学医学院病理教研室,湖北武汉430080 [2]武汉科技大学医学院肝病研究所 [3]北京大学人民医院肝病研究所

出　　处：《中西医结合肝病杂志》2004年第1期36-38,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的 :观察水蛭桃仁汤对肝纤维化大鼠肝星状细胞 (HSC)活化及转化生长因子 β1(TGF β1)表达的影响 ,并探讨其对肝纤维化是否具有治疗作用及其作用机制。方法 :以二甲基亚硝胺 (DMN)制备大鼠肝纤维化模型 ,同时给予水蛭桃仁汤灌胃治疗。免疫组化法检测肝组织的α 平滑肌动蛋白 (α SMA)和TGF β1,并做组织病理学检测。结果 :经图像分析 ,药物防治组α SMA和TGF β1较模型组显著减少 (均为P <0 0 1) ;结论 :水蛭桃仁汤对DMN诱导的实验性大鼠肝纤维化具有良好的防治作用。Objective: To study the effects of Decoction of leech and peach kernel on activation of HSC and expression of TGF-β1 in rat liver fibrosis and investigate the possible mechanisms of it in protection of liver fibrosis.Methods:The rat liver fibrosis model was induced by the intraperineal injection of Dimethylnitrosamine(DMN). At the same time, the treatment group was treated with Decoction of leech and peach kernel, the expression of α-SMA and TGF-β1 in liver tissue were detected by immunohistochemistry.Results:By an image analysis system, it showed that there were much less α-SMA and TGF-β1 in the liver tissue in treatment group than that in the control group(P<0.01). Conclusion:Decoction of leech and peach kernel showed good effects on the treatment of hepatic fibrosis induced by DMN.

关 键 词：水蛭桃仁汤 肝纤维化 大鼠 HSC TGF-Β1 中医药疗法 免疫组化法 药效学 

分 类 号：R285[医药卫生—中药学]