火菇素蛋白的免疫印迹检测  

DETECTION OF FLAMMULIN WITH IMMUNOBLOT

作　　者：周凯松[1] 吕鹏[1] 薛久刚[1] 张晗星[1] 陈畅[1] 张长铠[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100

出　　处：《微生物学通报》2004年第1期10-13,共4页Microbiology China

基　　金：教育部博士点基金资助项目 (No.2 0 0 0 0 42 2 1 2 )

摘　　要：从金针菇子实体浸提液中提取了抗癌活性物质火菇素 ,将纯化的火菇素用Freund佐剂乳化后注射到新西兰白兔体内 ,经数次加强免疫后采血并分离了抗血清 ,并以抗血清为探针建立了火菇素蛋白的免疫印迹法定性检测方法。应用免疫学方法检测金针菇菌丝体和子实体 ,结果显示 ,只在金针菇子实体中发现与火菇素抗体相结合的抗原信号。Flammulin, an anti-tumor protein, was purified from the aqueous extract of basidiomes of Flammulina Velutipes. Purified flammulin emulsified with Freund's adjuvant was injected subcutaneously into New Zealand white rabbits. After several immune enhancements, these animals were bled and sera were separated. Antiserum against flammulin in Western blots were applied to determine if flammulin be present in the liquid state culture or fruiting body. The result showed that anti-flammulin serum could recognize the aqueous extract of fruiting body in SDS-PAGE gels under the reducing conditions, no flammulin was detected in mycelia of Flammulina Velutipes.

关 键 词：火菇素 金针菇 免疫检测 

分 类 号：Q819[生物学—生物工程]