双歧杆菌影响溃疡性结肠炎患者肠黏膜的研究  

The effects of bifidobacterium on the intestinal mucosa of the patients with ulcerative colitis

作　　者：崔海宏[1] 陈村龙[1] 王继德[1] 杨玉捷[1] 孙勇[1] 王亚东[1] 赖卓胜[1] 

机构地区：[1]第一军医大学附属南方医院消化科,广州510515

出　　处：《中华内科杂志》2003年第8期554-557,共4页Chinese Journal of Internal Medicine

基　　金：广东省自然科学基金资助项目 (0 10 62 1)

摘　　要：目的　研究双歧杆菌对溃疡性结肠炎 (UC)患者肠黏膜内核因子κB (NF κB)的表达和激活的影响及肿瘤坏死因子 (TNF) α、白细胞介素 (IL) 1β、IL 10mRNA表达的影响。 方法　 30例UC患者经治疗后 ,应用Western蛋白印迹分析检测NF κBp6 5表达情况 ;用凝胶电泳迁移率改变分析检测NF κB与DNA结合活性 ;逆转录 PCR检测肠黏膜内细胞因子的表达。结果　应用双歧杆菌后 3例复发 ,对照组 14例复发 ,与对照组相比 ,NF κB与DNA结合活性明显降低 ,蛋白表达量明显下降 (治疗前 0 82± 0 0 5 ,治疗后 0 31± 0 0 5 ,P <0 0 1) ;双歧杆菌明显抑制了TNF α、IL 1β的表达 ;提高IL 10的表达。结论　双歧杆菌的作用可能与抑制NF κB的激活、降低TNF α、IL 1β等致炎因子的表达 ,提高ILObjective To investigate the effects of bifidobacterium(Bf)on the intestinal mucosa of the patients with ulcerative colitis(UC) Methods Thirty patients in clinical and endoscopic remission by sulphasalazine and glucocorticoid were randomized to receive either Bifid Triple Viable capsule(BIFICO), 420mg/d, or an identical placebo (starch) for 8 weeks Fecal samples were collected for stool culture before and after treatment Patients were assessed clinically endoscopically and histologically after 2 months or in the case of a relapse p65 and IκB expression was determined by Western blot analysis DNA binding activity of NF κB in colonic nuclear extracts was detected by electrophoretic mobility shift assay The mRNA expression of cytokines were identified by a semi quantitive assay, reverse transcription polymerase chain reaction Results Three patients in the BIFICO group had relapses within the 2 month follow up period, compared with 14 in the placebo group ( P <0 01) Fecal concentration of lactobacilli, bifidobacteria, increased significantly from baseline levels only in the BIFICO treated group ( P < 0 01) The expression of NF κB p65 and DNA binding activity of NF κB were significantly attenuated in the treat group than that in control( P <0 05) The mRNA expression of anti inflammatory cytokines elevated obviously comparable of control group Conclusions The Bf maybe impede the activation of NF κB, decrease the expression of TNF α and IL 1β and elevate the expression of IL 10 These results suggest that oral administration of this new probiotic preparation is effective in preventing flare ups of chronic UC It will become a prophylactic drug delaying the relapse of UC

关 键 词：双歧杆菌 溃疡性结肠炎 肠黏膜 细胞因子 Western蛋白印迹法 炎症 

分 类 号：R574.62[医药卫生—消化系统]