恶性黑色素瘤血管生成拟态的研究  

Study on vasculogenic mimicry in malignant melanoma

作　　者：孙保存[1] 张诗武[1] 赵秀兰[2] 郝希山[3] 

机构地区：[1]天津医科大学附属肿瘤医院病理科,天津医科大学病理教研室300060 [2]天津医科大学病理教研室 [3]天津医科大学附属肿瘤医院腹外科,300060

出　　处：《中华病理学杂志》2003年第6期539-543,共5页Chinese Journal of Pathology

基　　金：国家自然科学基金资助项目 (3 0 0 70 3 78)

摘　　要：目的　探讨在恶性黑色素瘤中是否存在肿瘤细胞通过自身变形并模拟产生血管样通道而获得自身血液供应。方法　收集恶性黑色素瘤中高度恶性组和低度恶性样本各 30例 ,制作成组织芯片 ,进行CD34免疫组织化学染色和PAS双重染色 ,比较这两组肿瘤中CD34和PAS阳性管道个数 ,以其差异研究血管生成拟态。结果　恶性黑色素瘤中高度恶性组CD34和PAS阳性计数之间的差别具有统计学意义 (P <0 0 1)。而低度恶性组CD34和PAS阳性计数之间的差别没有统计学意义 (P >0 0 5 )。结论　在高度恶性黑色素瘤中存在血管生成拟态 ,即肿瘤细胞模拟机体血管生成而产生血液通路。肿瘤细胞通过该通路获得营养供应以满足其生长和扩散的需要 ,从而获得较高的侵袭能力和恶性度。Objective To investigate the mode of angiogenesis between highly invasive malignant melanoma and poorly invasive malignant melanoma by immunohistochemistry and periodic acid-Schiff stain (PAS) and to discuss whether the tumor cells in highly invasive malignant melanoma carry vasculogenic mimicry through self-metamorphosis, thus acquiring blood supply to sustain their growth. Methods Thirty cases of highly invasive malignant melanoma and 30 cases of poorly invasive malignant melanoma were retrieved and reprocessed as tissue microarray for further investigations. The tissue microarray sections were then stained with CD34 and PAS; and the positivity rates were compared. Results There was a significant difference between CD34 and PAS staining in highly invasive malignant melanoma ( P <0.01). The difference was not statistically significant in poorly invasive malignant melanoma ( P >0.05). Conclusion Vasculogenic mimicry exists in some cases of highly invasive malignant melanoma. It is possible that the tumor cells can acquire blood supply to sustain growth and metastasize via this mechanism.

关 键 词：恶性黑色素瘤 血管生成 肿瘤细胞 免疫组织化学 肿瘤侵袭 肿瘤生物学 

分 类 号：R73-3[医药卫生—肿瘤]