外照射对体外培养的小细胞肺癌细胞系药物敏感性的影响  

INFLUENCE OF FRACTIONED RADIATION ON THE SENSITIVITY OF NCI-H446 CELL LINE TO CHEMOTHERAPEUTIC DRUG

作　　者：段蕴铀[1] 宋鹏[1] 聂青[1] 黄友章[1] 周丽君[1] 

机构地区：[1]海军总医院,北京100037

出　　处：《解放军医学杂志》2003年第7期624-626,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：观察分次外照射对小细胞肺癌NCI H44 6细胞系药物敏感的影响并探讨其机制。采用GWGP80型远距离6 0 Co治疗机对进入指数生长期的NCI H44 6细胞进行分次外照射 ,每次 2Gy,总剂量为 5 0Gy。通过不同浓度丝裂霉素的干扰来观察外照射前后NCI H44 6细胞存活率的变化。结果显示 ,在相同浓度丝裂霉素的干扰下 ,照射组细胞的存活率均明显高于未照射组 (P <0 0 1)。提示对小细胞肺癌细胞系NCI H44 6进行外照射可抑制其总RNA的生物合成 ,提高其MDR1mRNA的表达水平 ;The aim of this study was to investigate the effect of fractioned radiation on the sensitivity of NCI-H446 small cell lung cancer cell line to chemotherapeutic drug and its mechanism. Exponentially growing NCI-H446 cells were exposed to 50 Gy radiation which was administered in 25 fractions with 2 Gy per fraction. The survival rate of NCI-H446 cell line was observed after the interference of different concentration of Mitomycin C given before and after fractioned radiation treatment. The survival rate of the radiated cells was higher than that of the unradiated cells with the same concentration of Mitomycin C(P<0.01). Irradiation treatment to the NCI-H446 small cell lung cancer cell line could inhibit the synthesis of its total RNA, enhance the expression of its MDR 1 gene and obviously decrease the sensitivity of it to cancericidal drug.

关 键 词：肺肿瘤 放射疗法 RNA 药物敏感性 丝裂霉素 

分 类 号：R734.205[医药卫生—肿瘤]