肥胖儿童Apo E基因多态性与其血脂PCR-RFLP分析  

The PCR-RFLP Analysis to the Apolipoprotein E Polymorphism in Children with Simple Obesity,and the Association of the Genotype with the Lipid Metabolism

作　　者：刘长云[1] 孙未 刘红英[1] 李伟华[1] 季加芬[1] 张培森[1] 

机构地区：[1]潍坊医学院附属医院,山东潍坊261031 [2]青岛市儿童医院

出　　处：《潍坊医学院学报》2003年第5期329-331,共3页Acta Academiae Medicinae Weifang

基　　金：山东省卫生厅科研基金资助课题 (课题编号 :2 0 0 1CA1CCB8)

摘　　要：目的　探讨单纯性肥胖儿童载脂蛋白 (Apo)E基因多态性的分布及其对血脂、脂蛋白、载脂蛋白的影响。方法　选择 6～ 14岁单纯性肥胖儿童 4 0例和健康儿童 4 2例。受试者禁食 12h ,抽取外周静脉血 ,测定血清中甘油三酯 (TG) ,总胆固醇 (TC) ,高密度脂蛋白胆固醇 (HDL C) ,低密度脂蛋白胆固醇 (LDL C) ,载脂蛋白A1(ApoA1) ,载脂蛋白B10 0 (ApoB10 0 )浓度。应用改良的聚合酶链式反应 -限制性片段长度多态性 (PCR RFLP)分析及聚丙烯酰胺凝胶电泳方法对单纯性肥胖及健康儿童ApoE基因型进行分析。结果　本实验检出 4种ApoE基因型 ,健康及肥胖儿童ApoE等位基因频率分布不均 ,以ε3 为最常见。与健康儿童比较 ,肥胖儿童ε4等位基因频率增高 ,差异有显著性 (P <0 .0 5 )。ApoE基因多态性影响健康及肥胖儿童的血脂水平。结论　单纯性肥胖儿童有ApoE基因多态性的变化 ,且明显影响小儿血浆脂类代谢。Objective To investigate the distribution of apolipoprotein(apo)E polymorphism and the association of Apo E genotypes with plasma lipids lipoprotein and apolipoprotein in children with simple obesity,and to provide a theoretical basis for the prevention of CHD.Methods 40 children with simple obesity and 42 healthy children from 6 to 14 years old were chosen as obesity group and control group.Serum triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),apolipoprotein A_1(Apo A_1),apolipoprotein B_ 100 (Apo B_ 100 )were measured after they fasted for 12 hours.Polymerase chain reaction-restricted fragments length polymorphism(PCR-RFLP),polyacrylamide gel electrophoresis,and silver staining technique were used for the detection of apolipoprotein E genotype.Results Four genotypes of Apo E were detected,and their distribution was imbalanced.Most of them were ε_3 alleles.Compared with the control group,the frequency of ε_4 allele in obesity group was higher(P<0.05).Apo E polymorphism may affect plasma lipid levels of healthy and obesity children.Conclusion Children with simple obesity have different patterns of Apo E polymorphism compared with healthy children.Apo E polymorphism may affect plasma lipids metabolism.

关 键 词：胆固醇 载脂蛋白E 等位基因 肥胖症 儿童 

分 类 号：R723.14[医药卫生—儿科]