羟甲芬太尼对慢性缺氧大鼠呼吸功能的影响  

Effects of ohmefentanyl on respiratory function of chronic hypoxic rats

作　　者：许欣[1] 李强[1] 孙学军[2] 张殿尧[1] 王成海[2] 

机构地区：[1]第二军医大学长海医院呼吸内科,上海200433 [2]第二军医大学基础医学部神经生物学教研室

出　　处：《第二军医大学学报》2004年第2期168-171,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金 ( 3 93 0 0 0 60 )

摘　　要：目的 :探讨 μ阿片受体激动剂羟甲芬太尼 (OMF)对慢性缺氧大鼠呼吸功能的影响。 方法 :将 86只雄性 SD大鼠随机分为正常组和慢性缺氧组 ,慢性缺氧模型由经尾静脉 6次注射木瓜蛋白酶而建立。正常组大鼠侧脑室微量注射 OMF(n=1 0 )和同体积人工脑脊液 (a CSF) (n=1 0 )。慢性缺氧组大鼠又随机分为 :(1 )侧脑室组 ,分别微量注射阿片受体拮抗剂纳络酮(NL X) (n=1 2 )和 OMF(n=1 2 ) ,同时另有两组均注射 a CSF(n=1 2 )作为对照 ;(2 )孤束核组 ,分别微量注射 OMF(n=9)和同体积 a CSF(n=9)。在注射后 5、1 5、30、4 5、6 0 min测定各组大鼠呼吸频率 (RR)和潮气量 (VT)。 结果 :正常大鼠侧脑室注射OMF后 1 5、30、4 5、6 0 min,RR、VT 均显著下降 (P<0 .0 5 ) ;慢性缺氧大鼠侧脑室及孤束核注射 OMF后 1 5、30、4 5、6 0 m in,RR、VT 均显著降低 (P<0 .0 1 ) ,侧脑室注射 NL X后 1 5、30、4 5、6 0 m in,RR、VT均显著增高 (P<0 .0 5 )。结论 :μ阿片受体激动剂可能通过中枢相应受体降低 RR、VT 等呼吸指标 。Objective:To assess the effects of ohmefentanyl(OMF),a potent and selective agonist for μ opiate receptor,on respiratory function in chronic hypoxic rats.Methods:Totally 86 healthy male SD rats were randomly divided into the normal group ( n =20) and chronic hypoxic model group( n =66).The chronic hypoxic rat model was established by intravenous injections of papain once a week for 6 times.Rats in normal control group received OMF injection( n =10) and artificial cerebrospinal fluid(aCSF) injection( n =10).Rats in chronic hypoxic group were further divided into cerebroventricular administration group( n =48) and nucleus tractus solitari(NTS) administration group( n =18).Rats in cerebroventricular administration group received naloxone( n =12) and OMF( n =12),and the other 24 rats were taken as control.Rats in NTS group received OMF( n =9) and aCSF( n =9).The respiratory rate(RR) and tidal volume(V T) were determined 5,15,30,45 and 60 min after injection.Results:Intracerebroventricular administration of OMF in normal rats resulted in a significant decrease in RR and V T ( P <0.05) 15,30,45 and 60 min after injection.The microinjection of NLX in chronic hypoxic rats resulted in a marked increase in RR and V T( P <0.05).Cerebroventricular and NTS microinjection of ohmefentanyl in chronic hypoxic models resulted in a severe respiratory depression 15,30,45 and 60 min( P <0.01) after injection.Conclusion: It is indicated that the μ opiate receptor agonist has inhibition effects on respiratory function in chronic hypoxic rats through central opiate receptor.

关 键 词：羟甲芬太尼 慢性缺氧 大鼠 呼吸功能 μ阿片受体激动剂 

分 类 号：R364.4[医药卫生—病理学] R974[医药卫生—基础医学]