重症急性胰腺炎大鼠肥大细胞的改变  

Changes of mast cells during severe acute pancreatitis

作　　者：张敏敏[1] 屠振兴[1] 李兆申[1] 龚燕芳[1] 满晓华[1] 曹冬梅[1] 金晶[1] 

机构地区：[1]第二军医大学长海医院消化内科,上海200433

出　　处：《第二军医大学学报》2004年第2期157-160,共4页Academic Journal of Second Military Medical University

基　　金：军队临床医学高新技术重大项目 (后卫 -2 0 0 2 -2 0 ) ;国家自然科学基金 ( 3 0 2 70 5 0 4)

摘　　要：目的 :探讨肥大细胞 (MC)在重症急性胰腺炎 (SAP)发病过程中的作用。 方法 :将 1 5只健康雄性 SD大鼠随机分为3组 :假手术组、SAP3h组和 SAP6 h组 ,每组 5只。采用胆管注射 5 %牛磺胆酸钠法诱发 SAP,通过甲苯胺蓝染色以及 West-ern印迹方法观察 SAP大鼠胰腺组织 MC数量及活性的变化。 结果 :SAP3h组 ,胰腺 MC密度为 (6 .5 5± 1 .0 6 )个 /视野 (×2 0 0 ) ,较假手术组 [(2 .6 0± 0 .1 6 )个 /视野 ]明显增多 (P<0 .0 5 ) ;SAP6 h组的胰腺 MC密度为 (2 .2 0± 0 .5 2 )个 /视野 ,与假手术组无显著性差异 ,但较 SAP3h组明显降低 (P<0 .0 5 )。假手术组 MC类胰蛋白酶的条带灰度为 1 0 6 .3± 5 .6 ,SAP3h组MC类胰蛋白酶条带灰度为 1 85 .4± 3.6 ,较假手术组明显增高 (P<0 .0 5 ) ;SAP6 h组胰腺组织的 MC类胰蛋白酶的条带灰度为 1 6 6 .5± 6 .2 ,较假手术组明显增高 (P<0 .0 5 ) ,但与 SAP3h组无明显差异。 SAP3h组的胰腺病变评分为 6 .4± 0 .5 ,SAP6 h组为 1 2 .0± 0 .5 ,均较假手术组 (0 .0± 0 .0 )明显增高 (P<0 .0 5 )。胰腺组织的病理改变随着时间的延长而加剧。结论 :MC数量及活性变化是 SAP的早期事件 ,参与Objective:To study the pathological change and possible role of mast cells (MC) during the course of severe acute pancreatitis (SAP). Methods: Fifteen healthy male rats were randomized into 3 groups: control group( n =5), SAP 3 h group( n =5) and SAP 6 h group( n =5). SAP was induced by the intraductal infusion of 5% sodium taurodeoxycholate into the rats. The toluidine blue staining and Western blot method were used to observe the quantity and activity change of MC. The pancreatic tissue damage was also evaluated histologically. Results: MC significantly increased to 6.55±1.06/field of vision at 3 h after the induction of SAP, which was much higher than that of control. While at 6 h after the induction, the density of MC decreased significantly to 2.20±0.52/field of vision, which was obviously lower than that of the 3 h group. The gray degree of tryptase strap in the tissue of pancreas was 185.4±3.6 in the SAP 3 h group and 166.5±6.2 in the SAP 6 h group, and there were no significant differences between the 2 groups, while both were higher than that of the control group( P <0.05). The pathological score of SAP 3 h group (6.4±0.5) and SAP 6 h group(12.0±0.5) were both higher than that of the control( P <0.05). And the damage of pancreas aggravated with the change of time. Conclusion: MC may increase significantly during the early stage of SAP, which may take part in the earlier period of the SAP progress.

关 键 词：重症急性胰腺炎 大鼠 肥大细胞 类胰蛋白酶 实验 

分 类 号：R576[医药卫生—消化系统] R-33[医药卫生—内科学]