实验性肺癌中蛋白激酶C的表达及意义  

The Expression of Protein Kinase C in Rat Model of Lung Squamous Carcinoma Induced by Methylcholanthrene

作　　者：粟多顺[1] 杨勤[1] 谢汝佳[1] 杨国雄[1] 

机构地区：[1]贵阳医学院病理生理学教研室,贵州贵阳550004

出　　处：《肿瘤学杂志》2004年第1期20-22,共3页Journal of Chinese Oncology

基　　金：贵州省卫生厅优秀科教人才基金资助

摘　　要：[目的]建立肺癌动物模型基础上研究蛋白激酶C(PKC)α在肺鳞癌组织中表达,探讨其在肺鳞癌发生发展中的可能作用。[方法]用肺叶支气管内灌注致癌物碘油溶液诱发大白鼠肺癌的方法建立大鼠肺鳞癌模型,以免疫组化方法研究PKCα在肿瘤组织、肿瘤周围组织及正常组织的表达。[结果]诱发的肺部肿瘤均为肺鳞癌,诱癌率达77.8%;肺鳞癌组织中PKCα表达明显增高,在诱癌组对侧肺组织表达也明显高于正常肺组织,分别为(24.08±2.93)%,(5.00±2.10)%,(1.96±0.29)%,差异有显著性(P<0.05)。[结论]肺癌组织中PKCα的异常高表达提示致癌剂可能通过活化PKCα介导的多个环节的信号转导而参与了肺鳞癌的发生、发展。To evaluate the significance of the expression of protein kinase C(PKC)αin lung squa-mous carcinoma induced by methylcholanthrene(MAC)in rats.Wistar rat model with lung squa-mous carcinoma was induced by MAC in iodized oil.The rats were sacrificed at the end of the12th week after MAC treatment.Immunohistochemistry method was used to explore the expression of PKCαin lung squamous carcinoma.The expression rate of PKCαin lung squamous carcinoma,cancer around tissues and normal lung tissues was(24.08±2.93)%,(5.00±2.10)%,(1.96±0.29)%,respectively.The expression of PKCαin lung squamous carcinoma was significantly higher than that in cancer around tissues and normal lung tis-sues(P<0.05).The expression of PKCαin peripheral lung tissues was also higher than that in normal lung tis-sues(P<0.05).[Conclusion]The high expression of PKCαin lung squamous carcinoma indicates that the acti-vated PKCα,may mediate several signal transduction routine,and participant carcinogenesis and development in lung squamous carcinoma.

关 键 词：肺鳞癌 蛋白激酶C 大鼠 甲基胆蒽 

分 类 号：R73-3[医药卫生—肿瘤] R734.2[医药卫生—临床医学]