水稻两个frr基因的结构、转录特性及同源性分析  

The Structural, Transcriptional and Homology Analysis of Two frr Genes in Rice

作　　者：胡欣[1] 胡昊[1] 洪国藩[1,2] 韩斌[1] 

机构地区：[1]中国科学院上海生命科学研究院国家基因研究中心 [2]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031

出　　处：《植物生理与分子生物学学报》2004年第1期105-114,共10页Journal Of Plant Physiology and Molecular Biology

基　　金：中国科技部;中科院;上海市科委共同资助。

摘　　要：对水稻中两个核糖体再循环因子同源基因OsfrrA 和OsfrrB进行了鉴定与分析。这两个单拷贝基因分别位于水稻的4号和7号染色体上,且在细胞器基因组中未发现同源序列。它们在不同组织中的转录特性及其蛋白质产物的N端特征提示其翻译产物会被各自转运并分别定位于线粒体和叶绿体中,而序列上的保守性及构成性的表达则表明它们在植物生长中扮演着重要的角色。它们与其它原核及真核RRF之间在基因结构及编码序列上的异同为内共生学说提供了新的证据,也揭示了RRF在分子进化研究方面所具有的潜在价值。Two rice (Oryza sativa subsp.japonica cv.Nipponbare) ribosome recyclingfactor genes—OsfrrA and OsfrrB had been iden-tified and characterized(Figs.1, 2 & 4)inthis study. The gene OsfrrA is located on chro-mosome 4 while OsfrrB on chromosome 7. Noother homologue is found in rice organellegenomes. Both genes are unique in rice genome(Fig.3)and constitutively expressed(Fig.5). The N-terminal character of their encodedprotein products suggests that the proteins aretransferred to mitochondrion and chloroplast re-spectively (Table 2, Fig.6) and carry out theirfunctions. The sequence conservation and theconstitutive expression profile of the two genesstrongly imply their indispensable role in plantgrowth. In addition, these sequences share phy-logenetic homology to some extent with otherprokaryotic and eukaryotic RRFs(Fig.7), provid-ing further evidence for the endosymbiotictheory, and implying the potential value of RRFsin molecular evolution research.

关 键 词：核糖体再循环因子 水稻 终止后复合体 半自主细胞器 

分 类 号：S511[农业科学—作物学]