人X染色体间期细胞遗传学研究  

Inter phase Cytogenetic Studies of Human X Chromosome

作　　者：裘宏琛[1] 程在玉[1] 高春生[1] 叶丽珍[1] 马绍武[1] 朱俊真 李洪云 刘凤喜 傅学文 

机构地区：[1]中国医学科学院基础医学研究所医学遗传室,北京100730 [2]河北省医学院医学遗传室 [3]北京医科大学第一附属医院妇产科遗传室

出　　处：《Acta Genetica Sinica》1992年第2期101-106,共6页

摘　　要：应用人X染色体α卫星DNA探针进行X染色体正常或异常个体的外周血淋巴细胞染色体和间期核的原位杂交,在R显带的中期分裂相上,绝大部分杂交颂粒位于X染色体着丝粒区(p11→q11);在间期核内则显现与X染色体数相一致的银颗粒簇,其中相当部分位于核边缘区。实验结果表明,用原位杂交来检测X染色体数目,比记数Barr小体的方法可靠。本文还就α卫星DNA探针在间期细胞遗传学方面广泛的应用做了讨论。The chromosome in situ hybridization with human X chromosome a satellite DNA probe (pBamX7) on human lymphocyte metaphases and interphase nuclei was performed for interphase cytogenetic studies. The individuals with numerical or structural abnormalities of X chromosome were studied. The results showed that the probe hybridized specifically to the centromeric region (p11→q11) of X chromosome. The number of silver grain clusters in interphase nuclei was correlated with that of X chromosome. Most of the clusters lacated near the nuclear membrane where innctive X chromarins (Barr-bodies) were usually found. The method of ascertaining the number of X chromosomes by in situ hybridization was much more reliable than that by counting the number of Barr-bodies. The modified R-banding technique was introduced and the significance of this work was also disscussed.

关 键 词：细胞遗传学 染色体 间期 

分 类 号：Q343.2[生物学—遗传学]