T-DNA插入产生的水稻迟抽穗突变体的遗传分析  

Genetic Analysis of a Heading-delayed Mutant by T-DNA Insertion in Rice (Oryza sativa L.)

作　　者：陈兆贵[1,2] 王江[3] 张泽民[1] 刘芳[1] 朱海涛[1] 宛新杉[3] 张景六[3] 张桂权[1] 

机构地区：[1]华南农业大学植物分子育种广东省重点实验室 [2]现在广东省惠州学院理学系工作,广东惠州516007 [3]中国科学院上海生命科学研究院植物生理生态研究所植物分子遗传国家重点实验室

出　　处：《植物生理与分子生物学学报》2004年第1期75-80,共6页Journal Of Plant Physiology and Molecular Biology

基　　金：国家基础研究发展规划项目(G19990116)资助。~~

摘　　要：在筛选和鉴定水稻T-DNA插入纯合体的过程中,观察到一个迟抽穗的突变体。对该突变体进行遗传分析的结果表明,分离群体后代中出现正常抽穗、迟抽穗和特迟抽穗3种类型,分离比率为1∶2∶1,符合一对基因的不完全显性遗传;对突变体及其后代分离群体做Basta抗性检测及PCR分子检测,证实该突变体是由T-DNA插入所引起的,突变性状与T-DNA共分离。该材料可用于插入座位的基因克隆。Transposon tagging was used to iso-late genes in higher plant. In this study, a de-layed heading mutant caused by T-DNA inser-tion in rice was identified. Genetic analysis ofthe mutant showed that the three types ofphenotype, normal heading, delayed heading andoverly delayed heading (Table 1, Figs.1 and 2)in the segregating populations derived from theT-DNA heterozygotes fit the ratio of 1:2:1(Table2). Test for Basta resistance showed the delayedheading plants were all resistant while the nor-mal heading plants were susceptible, and the ra-tio of resistant and susceptible plants was 3:1(Table 3), which indicated that the delayed head-ing mutant was co-segregated with Basta resis-tance (Table 4). The delayed heading mutantcaused by T-DNA insertion was confirmed by T-DNA detection using PCR method (Fig.3, Table5). This delayed heading mutant will be used forisolation of the tagged gene in rice.

关 键 词：水稻 生育期 突变体 T—DNA插入 

分 类 号：S511[农业科学—作物学]