肺癌患者外周血中CK19-mRNA表达的检测  

Detection of Cytokeratin 19 mRNA in Peripheral Blood of Patients with Lung Cancer

作　　者：胡跃[1] 李旭芬[1] 张苏展[1] 

机构地区：[1]浙江大学医学院附属第二医院肿瘤科,310009

出　　处：《实用癌症杂志》2004年第1期40-42,46,共4页The Practical Journal of Cancer

摘　　要：目的　检测肺癌患者外周血中CK 19 mRNA的表达情况。方法　采用逆转录PCR (RT PCR )方法 ,分别检测有明确肿瘤病灶的肺癌患者、肺部良性疾病患者和健康对照者外周血中CK 19 mRNA的表达。结果　 70例肺癌患者外周血中CK 19 mRNA表达阳性率为 3 7.14 % ,3 8例肺部良性疾病患者阳性率为 18.42 % ,3 7例健康对照者阳性率为 5 .41% ,肺癌组与肺部良性疾病组、健康对照组间阳性率均有显著性差异 (P <0 .0 5 ) ,CK 19 mRNA表达阳性率与肿瘤分期及病理类型无相关性。结论　外周血中CK 19 mRNA作为肺癌的 1项分子检测指标 ,具有一定的临床应用价值 ,值得进一步扩大样本研究及随访 。Objective To investigate the CK19-mRNA expression in peripheral blood and its significance in patients with lung cancer.Methods Reverse transcriptase polymerase chain reaction(RT-PCR) was used to amplify the CK19-mRNA in blood samples from patients with lung cancer,benign lung diseases,and healthy volunteers.Results The positive rates of CK19-mRNA in peripheral blood were 37.14% in patient with lung cancer,18.42% in patients with benign lung diseases and 5.41% in healthy volunteers,showing a significant difference between lung cancer and begign lung diseases,and between lung cancer and healthy controls(P<0.05).The positive rate had no significant correlation with the TNM staging and histopathologic types.Conclusion CK19-mRNA in peripheral blood could be as a valuable molecular marker of lung cancer if its sensitivity and specificity are improved.Further studies with more samples and long-term follow-up are needed.

关 键 词：肺癌 外周血 CK19—mRNA 表达 检测 

分 类 号：R734.2[医药卫生—肿瘤]