中国海南岛3个黎族主要支系永生细胞系的建立与保存  

ESTABLISHMENT AND PRESERVATION OF IMMORTAL LYMP-HOBLASTOID CELL LINESOF THE HA-LI,THE QI-LI AND THE BENDI-LI ETHNIC GROUPSIN HAINAN ISLAND,CHINA

作　　者：李冬娜[1] 应大君[2] 区采莹[3] 陈路[3] 褚嘉祐[4] 薛雅丽[5] 

机构地区：[1]海南医学院生物学教研室,海口571101 [2]第三军医大学解剖学教研室,重庆400038 [3]海南医学院附属医院儿科,海口571101 [4]中科院医学生物研究所,昆明650107 [5]哈尔滨医科大学生物学教研室,哈尔滨150086

出　　处：《海南医学院学报》2004年第1期6-8,9,共4页Journal of Hainan Medical University

摘　　要：目的:建立侾黎、杞黎、本地黎3个黎族主要支系的永生细胞库。方法:采用EB病毒转化外周血B淋巴细胞并加环胞霉素A抑制T淋巴细胞建立永生细胞系。结果:成功地对侾黎、杞黎、本地黎的部分个体建立了永生细胞系。其中侾黎66株,杞黎76株,本地黎54株,共196株。结论:侾黎、杞黎、本地黎永生细胞库的建立使特有的黎族基因组得到系统地、永久地保存,并为分析其遗传学差异奠定了基础。Objective: This study was conducted to establish immortalized cell bank of the Ha-li,the Qi-li and the Benti-li ethnic groups in Hainan Island,China. Method: The immortal lymphoblastoid cell lines were established by Epstein-Barr virus transformation of B cells and addition of cyclosporin A to inhibit the activity of T cells. Res ults: In the present study,196immortal cell lines of the Ha-li,the Qi-li and the Bendi-li ethnic groups in Hainan Island were established. Conclus ion: The establishment of immortal cell lines of the three primary Li subgroups not only systematically and permanently preserves the special Li ethnic group genome but also provides enough research materials for further studies.

关 键 词：海南 黎族 永生细胞系 细胞库 遗传学 少数民族 EB病毒 

分 类 号：Q347[生物学—遗传学]