大肠癌P_(53)蛋白与增殖细胞核抗原的免疫组织化学研究  

An Immunohistochemistric Research on P_(53) Protein and Proliferating Cell Nuclear Antigen in Colorectal Cancer

作　　者：王桂华[1] 曹宁殊[1] 

机构地区：[1]嘉兴学院医学院附属二院,浙江嘉兴314000

出　　处：《浙江预防医学》2004年第3期13-14,共2页Zhejiang Journal of Preventive Medicine

摘　　要：目的　探讨P53蛋白、增殖细胞核抗原 (PCNA)在大肠癌细胞中的表达及临床病理和预后的关系。方法　应用免疫组化技术对 48例大肠癌组织进行P53、PCNA检测。结果　P53、PCNA阳性表达率分别为5 6%、 93 8% ,PCNA强阳性表达率达 66 7% ;P53阳性表达率及PCNA强阳性表达率与大肠癌组织类型、浸润深度、淋巴结转移密切相关 (P <0 0 5 )。结论　联合检测P53、PCNA对判断大肠癌恶性程度、预测其淋巴结转移趋势及判断预后有有重要价值。Objective To clarify the relation between the expression of P 53 protein and proliferating cell nuclear antigen (PCNA) in colorectal cancer cells and its clinical pathology and prognosis. Methods P 53 protein and PCNA were detected in 48 patients with colorectal cancer by immunohistochemistric technique. Results The positive rates of expressing P 53 protein and PCNA were 56.3% and 93.8% respectively, and the strong PCNA-positive rate was 66.7%, which were correlated with the histological type, invasion depth and lymph node metastasis significantly (p<0.05).Conclusions It is significant in combining detection of P 53 protein and PCNA for evaluating malignant degree, predicting lymph node metastasis trend and prognosis.

关 键 词：大肠癌 P53蛋白 增殖细胞核抗原 免疫组织化学 预后 肿瘤转移 

分 类 号：R735.37[医药卫生—肿瘤]