β-连环蛋白反义cDNA对肝癌细胞恶性表型的影响  

The inhibition of the malignant phenotype of human hepatic carcinoma cell line by antisense β-catenin cDNA

作　　者：范恺谊[1] 尹淼[1] 朱晓群[1] 徐莺莺[1] 申宗侯[1] 

机构地区：[1]复旦大学上海医学院生物化学与分子生物学系,上海200032

出　　处：《中国癌症杂志》2004年第1期1-4,共4页China Oncology

基　　金：国家自然科学基金项目 (No .39970 338);上海市教委重点项目 (No .8990 80 6 )

摘　　要：目的 :探讨应用反义核酸技术降低细胞内 β 连环蛋白水平对人肝癌SMMC 772 1细胞恶性表型的影响。方法 :构建 β 连环蛋白反义cDNA重组质粒 ,转染人肝癌SMMC 772 1细胞 ,筛选 β 连环蛋白低表达株 ,研究其恶性细胞表型的变化。结果 :转染 β 连环蛋白反义质粒后 ,低表达 β 连环蛋白的SMMC 772 1中c myc基因表达降低 ,细胞形态向未转化方向变化 ,细胞生长受到抑制 ,软琼脂克隆形成率下降 ,细胞周期发生改变 ,G0 G1期增加 ,S期减少。结论 :降低 β 连环蛋白表达可显著抑制 772Purpose:To study the effect of blocking β-c at enin expression on the malignant phenotypes of human hepatic carcinoma cell line SMMC-7721 by antisense ribonucleotide technique. Methods:The recombinant plasmid containing antisense β-catenin cDNA was constructed and tr ansfected into SMMC-7721. The malignant phenotypes of SMMC-7721 were analyzed by cell growth, potential colony formation in soft agar and cell cycle. Results:The morphology of SMMC-7721 cells with reduction of β-catenin gene expression level showed the appearance of nontransformed epithelial cells. Their growth ra te and colony-formation ability in soft agar were inhibited . Cell cycle analys is by flow cytometry showed that increase in cell population in the G 0 -G 1 phrase was associated with an arrest of S phase. C-myc mRNA, a target g ene regulated by β-catenin/TCF complex, was down-regulated in the AS-7721 ce lls. Conclusions:These results suggest that reducing β-catenin can partially reverse malignant phenotypes of human hepartic carcinoma cell line.

关 键 词：β—连环蛋白 反义核酸 肝肿瘤 培养的肿瘤细胞 

分 类 号：R735.7[医药卫生—肿瘤]