EB病毒基因BARF1在鼻咽癌细胞中的表达及意义  

Expression of Epstein-Barr virus gene BARF1 in the nasopharyngeal carcinoma and its significance

作　　者：张蕾[1] 王晓华[1] 马秀兰[2] 何安光[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所,辽宁沈阳110001 [2]中国医科大学附属第一医院耳鼻喉科,辽宁沈阳110001

出　　处：《中国癌症杂志》2004年第1期28-30,共3页China Oncology

基　　金：教育部留学归国人员科研启动基金(No 2 0 0 2 2 4 7)

摘　　要：目的 :探讨EB (Epstein Barr)病毒新基因BARF1(BamHIArightwardopenreadingframe 1)在人鼻咽癌组织中的表达及意义 ,为深入阐明EB病毒致癌机制提供实验依据。方法 :提取RNA后 ,采用RT PCR方法扩增标本中的EBNA1(EBvirusassociatednuclearantigen 1)和BARF1mRNA ,PCR产物经 2 %琼脂糖凝胶电泳观察并照相。结果 :11例RNA合格的标本均表达EBNA1,提示病例均为EB病毒阳性病例 ;其中 9例表达BARF1,占 82 % ;而且 9例中的 7例为强阳性。结论 :EB病毒新基因BARF1mRNA在鼻咽癌细胞中高表达 ,这提示除了已经明确的潜伏性膜蛋白 1(LMP1)以外 ,BARF1可能在鼻咽癌细胞恶性增殖中发挥重要作用 。Purpose:To study the expression of Epstein-Barr virus gene BARF1 in nasopharyngeal carc inoma cells, to uncover the mechanism and effects of Epstein-Barr virus in the pathogenesis of EBV related carcinomas, and to offer some experimental supports for the prevention and treatment of EBV related carcinomas Methods :After RNA extraction from nasopharyngeal carcinoma samples, RT-PCR was con ducted to amplify the EBV gene EB virus associated nuclear antigen-1 (EBNA1) an d the new gene Bam HI A open reading frame 1 (BARF1) Then the PCR products wer e electrophoresed by 2% agarose gel and the product bands were detected and phot ographed Results:Of the 13 samples, 11 were qualified for the RNA qualit y And all of the 11 NPC cases were EBNA1 positive, showing the EB virus infect ion in the carcinoma cells 9 of the 11 cases were BARF1 positive, the positive rate was nearly 82% Conclusions:The EB virus gene BARF1 was highly expressed in the NPC samples This implies t hat apart from the latent membrane protein 1 (LMP1), BARF1 may play an important role in the pathogenesis of NPC The exact mechanisms of the BARF1 effects on the pathogenesis of NPC need to be studied further

关 键 词：鼻咽癌 Epstein—Barr病毒 BARF1基因 

分 类 号：R739.6[医药卫生—肿瘤]