HIV-1 gag基因和gp120基因转化番茄及转基因植株再生  

Transformation with Genes gag and gp120 from HIV-1 into Tomato and Regeneration of Transgenic Plant

作　　者：余云舟[1] 金宁一 王罡[1] 季静[1] 王萍[1] 李昌[1] 

机构地区：[1]解放军军需大学植物基因工程研究中心,军事兽医研究所长春130062 [2]军事兽医研究所,长春130062

出　　处：《中国生物工程杂志》2004年第2期37-40,共4页China Biotechnology

基　　金：国家植物转基因中试及产业化基地"专项课题 (J99 B 0 0 1)

摘　　要：构建了HIV 1的gag基因、gp1 2 0基因及gag gp1 2 0嵌合基因的植物双元表达载体。通过农杆菌介导法将HIV 1的gag基因、gp1 2 0基因和gag gp1 2 0嵌合基因导入番茄 ,获得抗性转化再生植株。PCR检测和Southern杂交鉴定目的基因已整合到再生植株基因组中 ,获得了转基因植株。GUS染色及Northern杂交结果表明目的基因已得到表达。本实验首次进行了HIV 1抗原基因转化番茄的研究 ,为利用番茄生产艾滋病新型口服疫苗打下了良好的基础。Plant expression vectors were constructed with genes gag and gp120 from HIV-1 and these plasmid vectors pBI121gag、pBI121gp120、pBI121gag-gp120 and pBI131Sgp120 were transformed into Agrobacterium tumefaciens(LBA4404 and EHA105).The leaves of four tomato cultivars as explants were transformed by Agrobacterium tumefaciens(LBA4404 and EHA105) harboring plasmids carrying genes gag,gp120 and gag-gp120 from HIV-1.Kanamycin-resistant transformants have been obtained in the experiment.It was demonstrated that genes gag,gp120 and gag-gp120 had been inserted into tomato genome after PCR and Southern blot analysis of regenerated plants.Gus staining and Northern blot results showed that foreign genes have expressed preliminarily.To our knowledge,this is the first report that genes which are antigen of HIV-1 was transformed into Tomato.It made the basic research for transgenic tomato as bioreactor to produce oral vaccines against HIV.;

关 键 词：番茄 GAG基因 gpl20基因 遗传转化 再生植株 

分 类 号：S641.2[农业科学—蔬菜学]