体外胆管癌细胞x线照射后细胞周期及放射敏感性变化  

Radiosensitivity and growth characteristics of filial generation from irradiated bile duct cancer cells

作　　者：刘宏[1] 王庆伟[1] 章晓梅[1] 程玉峰[1] 

机构地区：[1]山东大学齐鲁医院放疗科,山东济南250012

出　　处：《中国现代普通外科进展》2003年第3期156-158,共3页Chinese Journal of Current Advances in General Surgery

摘　　要：目的 :探讨胆管癌细胞经x线照射后存活后代生长特性及放射敏感性的变化。方法 :人胆管癌细胞株和经x线照射后存活后代体外培养 ,测定其群体倍增时间、放射敏感性和细胞周期变化。结果 :胆管癌细胞的群体倍增时间为 (12 0 .0± 2 .8)h ,克隆形成率为 (14.0± 0 .6 ) %,放射敏感性参数SF2为0 .6 2± 0 .0 5。胆管癌细胞照射 6Gy后存活后代群体倍增时间为 (15 2 .8± 11.2 )h(t =3.12 4,P <0 .0 5 ) ,克隆形成率为 (6 .0± 0 .4) %(t=6 .5 2 2 ,P <0 .0 1) ,SF2为 0 .82± 0 .0 6 (t=2 .32 4,P <0 .0 5 )。胆管癌细胞x线照射后出现明显的S期细胞减少和G2 /M期阻滞 ,且有剂量依赖。结论 :胆管癌细胞x线照射后存活后代生长延缓 ,放射敏感性增高 ,呈S细胞减少和G2期阻滞。Objective:To investigate the changes of radiosensitivity and growth features of the surviving progeny from the irradiated bile duct cancer cells.Methods:To cultivate human bile duct cancer cell and the progeny of irradiated bile duct cancer,population doubling time(PDT),plating efficiency(PE),radiosensitivity index SF2 and the detection of bile duct cancer cell lines.Results:The PDT of bile duct cancer was (120.0±2.8)hrs,PE(14.0±0.6)%,SF2 0.62±0.05.The PDT of the irradiated bile duct cancer cells was(152.8±11.2) hrs (t=3.124,P<0.05),PE(6.0±0.4)%(t=6.522,P<0.01),SF2 0.82±0.06 (t=2.324,P<0.05).Bile duct cancer cell lines showed significant S reduce and G2 arrest,which was dose-dependent.Conclusion:In the present study,growth delay and enhanced radiosensitivity are confirmed in the progeny of irradiated bile duct cancer cells.Cell lines is showed significant S reduce and G2 arrest.

关 键 词：胆管瘤细胞 X线 细胞周期 放射敏感性 

分 类 号：R735.8[医药卫生—肿瘤] R730.55[医药卫生—临床医学]