从酵母表达时间序列估计基因调控网络  

Estimating Coarse Gene Networks from Yeast Gene Expression Time Series

作　　者：徐肖江[1] 王连水[1] 丁达夫[1] 

机构地区：[1]中国科学院上海生命科学研究院生物化学与细胞生物学研究所蛋白质组学重点实验室,上海200031

出　　处：《生物化学与生物物理学报》2003年第8期707-716,共10页

基　　金：国家高技术研究发展计划 (863计划)项目(No.2 0 0 2AA2 3 40 2 1);中国科学院知识创新工程项目(No.KJCX1 0 8);上海市科学技术委员会科技项目(No .0 0JC14 0 18)资助~~

摘　　要：基因调控网络是生命功能在基因表达层面上的展现。用组合线性调控模型、调控元件识别和基因聚类等方法 ,从基因组表达谱解读酵母在细胞周期与环境胁迫中的基因调控网络。结果表明 ,细胞在不同环境条件下会调整基因调控网络。在适应的环境下 ,起主要作用的是细胞生长和增殖有关的基因调控网络 ;而在响应环境胁迫时 ,细胞会再规划调控网络 ,抑制细胞生长和增殖相关的基因 ,诱导跟适应性糖类代谢与结构修复相关的基因 ,还可能启动减数分裂产生孢子。分别从细胞周期和环境胁迫响应相关基因中 ,搜索到转录因子Mcm1结合位点TT CC T GGAAA ,和Dal82在尿囊素代谢途径相关基因上的结合位点TGAAAAWTTT。从而 ,从酵母表达时间序列估计基因调控网络是可行的 。Gene networks is the collection of gene gene regulatory relations at the expression level. In this study, a combined approach of the linear transcriptional modeling, identification of promoter elements and gene co expression clustering is developed to decipher yeast gene networks from expression time series. The cell must reorganize the genomic expression to programs required for growth and survival in each environment. The expression of many genes is regulated by environmental stress. The products of many genes that induced in the environmental stress are involved in metabolism of carbohydrates, structural repairs and even sporulation. Interestingly, it is identified that transcription factors Mcm1 and Dal82 matched their binding sites TT CC T GGAAA and TGAAAAWTTT in cell cycle progression and environmental stress response, respectively. These conclusions agree with the known observations. The results indicate that the approach may be useful for modeling gene networks from microarray data.

关 键 词：基因调控网络 基因组表达时间序列 细胞周期 环境胁迫 

分 类 号：Q75[生物学—分子生物学]