水稻逆转录转座子RIRE10拷贝数与LTR区转录活性的鉴定  

Characterization of the Copy Number of RIRE 10 Retrotransposon and Transcriptional Activity of Its LTR in Rice Genome

作　　者：王嵘[1] 洪国藩[1] 韩斌[1] 

机构地区：[1]中国科学院上海生命科学研究院国家基因研究中心

出　　处：《生物化学与生物物理学报》2003年第8期768-773,共6页

基　　金：国家科技部基金资助;中国科学院基金资助;上海市科学技术委员会基金资助~~

摘　　要：在水稻第四号染色体的长臂上鉴定了一个结构完整的Ty3型逆转录转座子RIRE10。RIRE10两LTR间的中间区域在gag pol的上游还包含另一个开放阅读框。通过RT PCR与Northern印迹杂交检测到来自LTR区的转录产物 ;根据点杂交结果 ,鉴定出包含中间区域的RIRE10成员的个数以及LTR区的拷贝数。除了 6 5个完整的逆转录转座子所具备的两个LTR外 ,水稻基因组还含有近 90 0个RIRE10的solo LTR。LTR区的转录以及导致soloA full length Ty3 like retrotransposon, named RIRE 10 , was identified on the long arm of chromosome 4 in rice genome. The internal region between two LTRs had another open reading frame in the region upstream of gag pol sequence. The transcripts from LTR region were detected by Northern blot hybridization and RT PCR. To assess the activity of RIRE 10 in rice genome, the copy number of its internal region and long terminal repeat (LTR) domain were determined by dot blot analyses. Nearly 900 solo LTR of the RIRE 10 retrotransposon exist in rice genome, apart from those LTRs that flank 65 intact RIRE retrotransposons. Based on the experimental results, the retrotransposition of RIRE 10 was speculated to be influenced by two factors: transcriptional activity of LTR region and homologous recombination resulting in solo LTR.

关 键 词：栽培稻 逆转录转座子 转录 LTR区 水稻基因组 

分 类 号：S511[农业科学—作物学]