光学离焦性近视眼视网膜pax-6基因表达的研究  

Expression of pax-6 in retina of eyes with myopia induced by optical defocus

作　　者：钟兴武[1] 葛坚[1] 邓文国[1] 陈晓莲[1] 聂昊辉[1] 黄娟[1] 

机构地区：[1]中山大学中山眼科中心,广州510060

出　　处：《中华眼底病杂志》2003年第4期244-246,共3页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金资助项目 (30 1 0 0 2 0 5)

摘　　要：目的　研究幼猴光学离焦性近视眼发生、发展过程中视网膜 pax- 6基因的表达情况 ,探讨pax- 6基因是否参与近视眼的发生、发展和正视化过程。　方法　选用猴龄为 1～ 3个月的恒河猴 9只 ,采用配戴眼镜或施行角膜屈光手术的方法 ,造成幼猴视网膜光学离焦。用反转录聚合酶链反应及定量分析方法检测不同时间视网膜 pax- 6基因的表达 ,并与非光学离焦眼进行比较。　结果　远视性光学离焦导致幼猴玻璃体腔增长速度加快 ,形成近视眼 ,其视网膜 pax- 6基因表达量明显比正常对照眼高 (t=3.4 80 ,P=0 .0 0 4 )。　结论　远视性光学离焦幼猴的视网膜 pax- 6基因表达明显增加 ,表明 pax- 6基因可能参与视觉依赖的眼球生长和正视化过程。Objectives To investigate the expression of pax-6 in retina of infant monkeys with myopia induced by optical defocus, and to determine the role of pax-6 would play or not in onset and development of myopia and emmetropization. Methods Nine healthy infant rhesus monkeys, aged from 1 to 3 months, were selected and wore spectacle lenses or underwent photorefractive keratectomy (PRK).Transcription polymerase chain reaction method and quantitative analysis were used to determine the expression of pax-6 in the retina with myopia induced by optical defocus in different time, and the result was compared with that in retina without myopia. Results The myopia caused by hyperopic defocus was found. The expression of pax-6 in the retina with myopia induced by optical defocus was significantly higher than that in the retina without myopia(t=3.480,P=0.004). Conclusions The expression of pax-6 is enhanced by hyperopic defocus in the infant monkey retina, which suggests that pax-6 may be involved in vision-dependent eye growth and emmetropization.

关 键 词：光学离焦性近视眼 视网膜 pax-6基因表达 手术 

分 类 号：R774.1[医药卫生—眼科]