正常人骨髓成纤维样基质细胞系对白血病细胞U937增殖的影响  

Effects of normal human bone marrow fibroblastoid stromal cell line on proliferation of acute myeloid leukemia cell line U937

作　　者：梁蓉[1] 黄高升[1] 王哲[1] 冯骥良[1] 郭英[1] 杨国嵘[1] 

机构地区：[1]第四军医大学基础部病理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2003年第17期1560-1562,共3页Journal of the Fourth Military Medical University

摘　　要：目的 :观察正常人骨髓成纤维样细胞系HFCL对白血病细胞U937增殖的影响 .方法 :采用免疫组化对HFCL细胞进行鉴定 .建立U937细胞和HFCL细胞共培养体系 ,台盼蓝拒染法测定生长曲线 ;HE染色和瑞氏 吉姆萨染色后进行分裂指数 (MI)测定 ;流式细胞仪 (FCM)检测细胞周期的变化 ;WesternBlot检测增殖细胞核抗原 (PCNA)表达 .结果 :与单独U937细胞培养相比 ,与HFCL细胞共培养后 ,U937细胞生长受抑 ,且与HFCL细胞直接接触组的抑制作用大于用transwell组 .其分裂指数单独白血病细胞组 >用transwell组 >与HFCL直接接触组 .细胞周期分析发现U937细胞与HFCL细胞共培养后 ,G1期细胞增高 ,S期细胞减少 ,以直接接触组为甚 .与HFCL细胞共培养后 ,U937细胞的PCNA表达下调 ;而与U937细胞共培养 ,HFCL细胞生长和细胞周期无明显变化 .结论 :正常人骨髓成纤维样细胞HFCL能抑制白血病细胞U937的增殖 ,阻止U937细胞细胞周期的运行 。AIM: To investigate the effects of normal human bone marrow fibroblastoid stromal cell line on the proliferation of acute myeloid leukemia cell line U937. METHODS: Coculture system of leukemia cell line U937 and normal bone marrow fibroblastoid stromal cell line HFCL was established and the growth curves were detected by cell counting. Cells were taken for Wright Giemsa staining and the mitotic index (MI) was determined. Flow cytometry and Western Blot were used to study the changes of cell cycle and expression of proliferating cell nuclear antigen (PCNA) separately. RESULTS: Compared with that of U937 cells without HFCL cells, the proliferation of U937 cells in direct contact with HFCL cells and U937 cells with HFCL cells separated by transwell was inhibited. The MI of U937 cells without HFCL cells was the highest, followed by U937 cells separated by transwell and U937 cells in direct contact with HFCL cells. The expression of PCNA in U937 cells with HFCL cells was lower than that of U937 cells without HFCL cells. The percentage of G1 phase cells of U937 cells with HFCL cells was higher than that without HFCL cells and the percentage of S phase cells was lower. CONCLUSION: The normal bone marrow fibroblastoid stromal cells HFCL can inhibit the proliferation and progress of cell cycle of acute myeloid leukemia U937 cells.

关 键 词：骨髓 成纤维样细胞 白血病 增殖 

分 类 号：R733.7[医药卫生—肿瘤]