绿僵菌对草坪灰翅贪夜蛾和粘虫的毒力及田间防治效果  

Virulence of Metarhizium anisopliae to Spodoptera mauritia (Boisduval) and Leucania separata Walker and its field control effects

作　　者：陈斌[1] 李正跃[1] 桂富荣[1] 

机构地区：[1]云南农业大学植物保护学院,昆明650201

出　　处：《植物保护》2004年第1期32-36,共5页Plant Protection

基　　金：云南农业大学青年基金

摘　　要：测定了从草坪灰翅贪夜蛾上分离的绿僵菌(Metarhizium anisopliae)对灰翅贪夜蛾和粘虫的毒力,采用时间-剂量-死亡率模型分析了绿僵菌对灰翅贪夜蛾和粘虫的毒力。结果表明绿僵菌对灰翅贪夜蛾和粘虫的剂量效应常数分别为0.69和0.66;在孢子含量1×106-7个／mL高剂量下接种,灰翅贪夜蛾和粘虫幼虫在接种第7天和第8天累积死亡率达100％;在孢子含量1×103-4个／mL低剂量下,接种后第10天达到死亡高峰。在孢子含量1×102个／mL下的累积死亡率分别为6％和4％。绿僵菌对灰翅贪夜蛾和粘虫致死剂量是时间的函数,对灰翅贪夜蛾第5天和第7天的LC50为孢子含量2.1×106和3.5×104个／mL,在孢子含量107-105个／mL下的LT50分别为4.15、5.1、6.18、7.7 d;对粘虫第5天和第7天的LC50为孢子含量1.55×107、2.39×105个／mL,在孢子含量107-105个／mL下的LT50分别为4.4、5.4、6.4、7.8 d。在草坪禾草上喷洒绿僵菌孢子含量1×106个／mL,5、7、10 d后对粘虫的防治效果分别为46.4％、65.8％、71.4％,对灰翅贪夜蛾的防效分别为47.6％、66.0％和79.6％。绿僵菌制剂孢子含量为1×105个／mL对两种害虫的防治效果相近。Virulence of isolate Ma020815 of Metarhizium anisopliae to the larvae of Spodoptera mauritia (Boisduval) and Leucania separata Walker was assessed in the laboratory. A time - dose - mortality model was applied to analysis of the variation of mortality rates. The model showed that the isolate Ma020815 was a bit more virulent to S. mauritia than to L. separata . The accumulative mortality went up to 100 % at 7 and 8 day after inoculation with a dose of 1 × 107 and 1 × 106 conidia/mL for S. mauritia and L. separata , respectively, and the accumulative mortality was 6% and 4% for S. mauritia, L. separata respectively, under the dose of 1 × 102 conidia/mL. The LT50 ranged from 4.15to 7.7 days for S. mauritia but 4.4 to 7.8 days for L. separata under the dose of 1 × 107 and 1 × 105 conidia/ mL. Its field control effect on L . separata at 5th, 7th and 10th day was 46. 4 % , 65. 8 % and 71. 4 % , respectively. Its control effect on S. mauritia was 47.6% , 66.60% ,and 79.6% at 5th, 7th and 10th day accordingly. The control effect with the dose of 1 × 105 conidia/mL on L. separata and S. mauritia was nearly the same as that with the dose of 1 × 106 conidia/mL.

关 键 词：绿僵菌 草坪害虫 灰翅贪夜蛾 粘虫 毒力 田间防治试验 时间-剂量-死亡率模型 生物防治 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治] S476.12[农业科学—植物保护]