A mouse model of severe acute pancreatitis induced with caerulein and lipopolysaccharide  

作　　者：Shi-Ping Ding Ji-Cheng Li Chang Jin Department of Lymphology,Department of Histology and Embryology,Medical College of Zhejiang University,Hangzhou 310031,Zhejiang Province,China 

出　　处：《World Journal of Gastroenterology》2003年第3期584-589,共6页世界胃肠病学杂志（英文版）

摘　　要：AIM: To establish a non-traumatic, easy to induce and reproducible mouse model of severe acute pancreatitis (SAP)induced with caerulein and lipopolyasccharide (LPS).METHODS: Thirty-two healthy mature NIH female mice were selected and divided at random into four groups (each of 8 mice), i.e., the control group (NS group), the caerulein group (Ch group), the lipopolysaccharide group (LPS group),and the caerulein+LPS group (Cn+LPS group). Mice were injected intraperitoneally with caerulein only, or LPS only,and caerulein and LPS in combination. All the animals were then killed by neck dislocation three hours after the last intraperitoneal injection. The pancreas and exo-pancreatic organs were then carefully removed for microscopic examination. And the pancreatic acinus was further observed under transmission electron microscope (TEM). Pancreatic weight, serum amylase, serum nitric oxide (NO)concentration, superoxide dismutase (SOD) and malondialdehyde (MDA) concentration of the pancreas were assayed respectively.RESULTS: (1) NS animals displayed normal pancreatic structure both in the exocrine and endocrine. In the LPS group, the pancreas was slightly edematous, with the infiltration of a few inflammatory cells and the necrosis of the adjacent fat tissues. All the animals of the Cn group showed distinct signs of a mild edematous pancreatitis characterized by interstitial edema, infiltration of neutrophil and mononuclear cells, but without obvious parenchyma necrosis and hemorrhage. In contrast, the Cn+LPS groupshowed more diffuse focal areas of nonviable pancreatic and hemorrhage as well as systemic organ dysfunction.According to Schmidt's criteria, the pancreatic histologic score showed that there existed significant difference in the Cn+LPS group in the interstitial edema, inflammatory infiltration,parenchyma necrosis and parenchyma homorrhage in comparison with those of the Cn group, LPS group and NS group (P＜0.01 or P＜0.05). (2) The ultrasturcture of acinar cells was seriously damaged in the Cn+LPS group. ChAIM;TO establish a non-traumatic,easy to induce and reproducible mouse model of severe acute pancreatitis(SAP) induced with caerulein and lipopolyasccharide(LPS). METHODS:Thirty-two healthy mature NIH female mice were selected and divided at random into four groups(each of 8 mice),i.e.,the control group(NS group),the caerulein group(Cn group),the lipopolysaccharide group(LPS group), and the caerulein+LPS group(Cn+LPS group).Mice were injected intraperitoneally with caerulein only,or LPS only, and caerulein and LPS in combination.All the animals were then killed by neck dislocation three hours after the last intraperitoneal injection.The pancreas and exo-pancreatic organs were then carefully removed for microscopic examination.And the pancreatic acinus was further observed under transmission electron microscope(TEM).Pancreatic weight,serum amylase,serum nitric oxide(NO) concentration,superoxide dismutase(SOD)and malondialdehyde(MDA)concentration of the pancreas were assayed respectively. RESULTS:(1)NS animals displayed normal pancreatic structure both in the exocrine and endocrine.In the LPS group,the pancreas was slightly edematous,with the infiltration of a few inflammatory cells and the necrosis of the adjacent fat tissues.All the animals of the Cn group showed distinct signs of a mild edematous pancreatitis characterized by interstitial edema,infiltration of neutrophil and mononuclear cells,but without obvious parenchyma necrosis and hemorrhage.In contrast,the Cn+LPS group showed more diffuse focal areas of nonviable pancreatic and hemorrhage as well as systemic organ dysfunction. According to Schmidt's criteria,the pancreatic histologic score showed that there existed significant difference in the Cn+LPS group in the interstitial edema,inflammatory infiltration, parenchyma necrosis and parenchyma homorrhage in comparison with those of the Cn group,LPS group and NS group(P<0.01 or P<0.05).(2)The ultrasturcture of acinar cells was seriously damaged in the Cn+LPS group.Chromatin margination of nuclei was present

关 键 词：黄蛙素 脂多糖 动物模型 急性胰腺炎 

分 类 号：R576[医药卫生—消化系统]