槐定碱诱导人胃癌MGC-803细胞凋亡的实验研究  

Apoptosis of gastric carcinoma MGC-803 cells induced by sophoridine

作　　者：周炳刚[1] 苏刚[2] 马德强[2] 孙靖中[1] 范玉琢[2] 郝永存[2] 

机构地区：[1]山东大学齐鲁医院普外科,济南250012 [2]宁夏自治区人民医院普外科,750021

出　　处：《肿瘤》2003年第3期197-199,共3页Tumor

摘　　要：目的　探讨槐定碱对人胃癌MGC 80 3细胞抑制作用及其诱导凋亡机理。方法　用MTT法测定槐定碱对MGC 80 3细胞的IC50 ,荧光显微镜、电子显微镜、流式细胞仪和DNA凝胶电泳等技术观察细胞凋亡。结果　实验显示 0 .4～ 3.2mg /ml的槐定碱对体外培养的MGC 80 3细胞有明显的抑制作用并可诱导其发生凋亡 ,凋亡细胞表现为细胞固缩 ,核染色质聚集或碎裂、胞质空泡化等 ;DNA电泳可见DNA“梯形”条带 ;流式细胞仪检测Sub G1凋亡峰在G1期前出现 ,S期细胞比例增高。结论　槐定碱对体外培养MGC 80 3细胞生长有抑制作用 ,机理与通过阻止细胞周期的进程 ,诱导肿瘤细胞凋亡有关。ObjectiveTo investigate the growth-inhibition and the mechanism of apoptosis induced by sophoridine on MGC-803 cell line. MethodsMTT assay was used to detect the half-inhibition concentration (IC_ 50 ), Fluorescence microscopy, electron microscopy, flow cytometry (FCM) and DNA gel electrophoresis were used to demonstrate the presence of apoptosis. ResultsThe experiment showed that 0.4-3.2mg /ml sophoridine could inhibit the growth of MGC-803 cells significantly, and morphological characteristics of apoptosis was observed with condensation of nucleus, bubble of cytoplasm, fragment of nucleus, and a ladder-like pattern of DNA fragmentation in agarose gel electrophoresis. Sub-G_1 peak and increased S period cells were shown by FCM. ConclusionSophoridine can induce the inhibition of cell growth by means of apoptosis, and the blocking of S period cells is involved in the mechanism of apoptosis.

关 键 词：槐定碱 胃癌 MGC-803细胞 细胞凋亡 肿瘤 细胞培养 

分 类 号：R735.2[医药卫生—肿瘤]