有丝分裂抑制剂诱发小鼠骨髓细胞C-有丝分裂效应研究  

A STUDY OF C-MITOTIC EFFECTS BY MITOTIC ARRESTANTS IN MOUSE BONE MARROW CELLS

作　　者：合正基 周汝敏 曹能 汪旭 

机构地区：[1]云南师大生物系

出　　处：《云南师范大学学报（自然科学版）》1992年第3期89-92,共4页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家自然科学基金;云南省科委基金资助项目

摘　　要：以有丝分裂指数、C-有丝分裂细胞频率及有丝分裂后期频率三项指标评价了三种有丝分裂抑制剂(秋水仙素,COL;对苯二酚,HQ;昆明山海棠根部水抽提物,THH)在小鼠骨髓细胞中的C-有丝分裂效应。研究发现:THH具有与COL类似的C-有丝分裂效应,较强烈地阻止有丝分裂细胞进入后期:而HO则出现阻止细胞进入有丝分裂及阻断中期细胞进入分裂后期的细胞与遗传毒性。结果提示,THH及HO都可能具有与COL相似的诱发哺乳动物非整倍体的作用,C-有丝分裂效应分析能够作用检测哺乳动物非整倍体诱发剂的辅助手段。Three criteria were employed to assaay the C-Mitotic (CM) effects of the mitotic arrestants [Colchicine, COL;Hydroquinone, HQ;Tripterygium hypoglaucum (level) Hutch, THH] in mouse bone marrow cells. COLand THH showed strong positive in CMeffect tests: The mitotic Indexes and the frequencies of the mitosis wrer significantly increased. HQwas also positive in the tests, but it could not only block the metaphase to enter anaphase but also the promotation of the mitosis.The results indicated that HQ and THH may be colchicine-like aneuploidy inducers in mammalian cells in vivo.The CM effect test is a useful method to predetect suspected aneugens.

关 键 词：C-有丝分裂 有丝分裂 抑制剂 

分 类 号：Q253[生物学—细胞生物学]