酿酒酵母在硅橡胶膜生物反应器中连续发酵的生长动力学  

Growth kinetics of Saccharomyces cerevisiae in a silicone rubber membrane bioreactor by continuous fermentation

作　　者：伍勇[1] 肖泽仪[1] 黄卫星[1] 石尔[1] 

机构地区：[1]四川大学化工学院化工机械系,四川成都610065

出　　处：《现代化工》2004年第1期34-36,39,共4页Modern Chemical Industry

基　　金：国家自然科学基金资助项目(20176030)

摘　　要：实验研究了酿酒酵母在硅橡胶膜生物反应器中的细胞生长速率与操作参数之间的关系。在三水平正交实验基础上,用Gauss Newton非线性最小二乘法拟合了细胞比生长率与葡萄糖浓度、乙醇浓度和细胞浓度3变量之间的关系式,得到硅橡胶膜生物反应器乙醇连续发酵的细胞最大比生长率、饱和常数、产物抑制常数和群聚抑制常数等基本动力学参数,表明当细胞浓度达到15g/L或乙醇浓度达到70g/L时细胞生长受到完全抑制。连续发酵实验验证了硅橡胶膜生物反应器中的细胞生长动力学满足拟合模型的规律。The effects of operating conditions on the growth rate of Saccharomyces cerevisiae was experimentally studied in a silicone rubber membrane bioreactor.Dependence of the specific growth rate on glucose,ethanol and cell concentrations and the principal kinetic parameters were quantified by nonlinear least-square data fitting with Gauss-Newton method based on orthogonal three-level experiments.The results indicated that the cell growth would be totally inhibited when cell concentration reached 15 g/L or ethanol concentration reached 70 g/L.The continuous fermentation experiments proved that the kinetic behaviors of the yeast cell in the silicone rubber membrane bioreactor agreed with the fit models.

关 键 词：酿酒酵母 膜生物反应器 乙醇连续发酵 细胞生长 

分 类 号：TQ033[化学工程] Q27[生物学—细胞生物学]