彭泽鲫雌核发育的细胞学研究  

Cytological studies in Carassius auratus var pengze

作　　者：杨睿姣[1] 张轩杰[1] 

机构地区：[1]湖南师范大学生命科学学院,湖南长沙410081

出　　处：《水产学报》2004年第1期1-7,共7页Journal of Fisheries of China

基　　金：农业部"彭泽鲫倍性检测"项目资助

摘　　要：通过对彭泽鲫(♀)×彭泽鲫(♂)的受精细胞学观察,彭泽鲫卵子受精后,具有与两性生殖鱼类相同的精子入卵过程和排出极体,但精子入卵后精核始终不解凝,也不与雌性原核融合。对精母细胞成熟分裂中期染色体观察表明,彭泽鲫精母细胞在进行成熟分裂时未发生同源染色体配对,全部为单价体形式,其精子形成过程中第一次成熟分裂异常;对精子与体细胞DNA含量进行比较,体细胞DNA含量与精子DNA含量比为2.218:1,精子DNA含量发生了减半。以上实验结果说明彭泽鲫为雌核发育鱼类。同时,作者对卵核染色体倍性保持稳定的方式及精卵不同的形成机制进行了初步的分析。It was observed that fertilization cytology of Carassius auratus vat pengze (♀)×Carassius auratus var pengze (♂). It was observed that after being inseminated, a sperm entered egg through micropyle and a polar body was extruded. But after a sperm entered the egg, spermatozoon nucleus beside female pronucleus was condensed and cannot fuse with female pronucleus. The result shows that Carassius auratus var pengze is a gynogenetic reproductive species. There were no pairs of homologous chromosome in meiosis Ⅰ metaphase of spermatocyte of Carassius auratus var pengze. It shows the unusual meiosis Ⅰ during spermatogenesis. The ratio of DNA relative content of tail fin cell to sperm of Carassius auratus vat pengze is 2.218: 1, and it reveals that DNA relative content of sperm is half of somatic cell. During spermatogenesis, meiosis Ⅰ is abnormal. This paper preliminarily analyzed the mode of the stability of chromosome ploidy of Carassius auratus vat pengze and the different mechanism of ogenesis and spermatogenesis in order to better understand the reproductive modes and formation mechanism of triploid of Carassius auratus var pengze.

关 键 词：彭泽鲫 雌核发育 受精细胞学 成熟分裂 DNA相对含量 生殖方式 三倍体 形成机制 

分 类 号：S965.117[农业科学—水产养殖] Q959.468[农业科学—水产科学]