Loss of clusterin both in serum and tissue correlates with the tumorigenesis of esophageal squamous cell carcinoma via proteomics approaches  

作　　者：Li-YongZhang Wan-TaoYing You-ShengMao Hong-ZhiHe YuLiu Hui-XinWang FangLiu KunWang De-ChaoZhang YingWang MinWu Xiao-HongQian Xiao-HangZhao 

机构地区：[1]NationalLaboratoryofMolecularOncology,CancerInstituteandHospital,ChineseAcademyofMedicalSciences&PekingUnionMedicalCollege,Beijing100021,Beijing,China [2]DepartmentOfGenomicsandProteomics,BeijingInstituteofRadiationMedicine,Beijing100850,China [3]DepartmentofPectoralSurgery,CancerInstituteandHospital,ChineseAcademyofMedicalSciences&PekingUnionMedicalCollege,Beijing100021,Beijing,China [4]BeijingYanjingHospital,Beijing100037,China

出　　处：《World Journal of Gastroenterology》2003年第4期650-654,共5页世界胃肠病学杂志（英文版）

基　　金：the Major State Basic Research Development Program of China,No.G19980512 and No.2001CB510201;the National Hi-Tech R & D Program of China,No.2001AA227091 and No.2001 AA233061;National Natural Science Foundation of China,No.39990570,No.30171049,30225045 and No.39990600

摘　　要：AIM: To identify the differentially secreted proteins or polypeptides associated with tumorigenesis of esophageal squamous cell carcinoma (ESCC) from serum and to find potential tumor secreted biomarkers.METHODS: Proteins from human ESCC tissue and its matched adjacent normal tissue; pre-surgery and postsurgery serum; and pre-surgery and normal control serum were separated by two-dimensional electrophoresis (2-DE)to identify differentially expressed proteins. The silverstained 2-DE were scanned with digital ImageScanner and analyzed with ImageMaster 2D Elite 3.10 software. A cluster of protein spots differentially expressed were selected and identified with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). One of the differentially expressed proteins, clusterin, was downregulated in cancer tissue and pre-surgery serum, but it was reversed in post-surgery serum. The results were confirmed by semi-quantitative reverse-transcription (RT)-PCR and western blot.RESULTS: Comparisons of the protein spots identified on the 2-DE maps from human matched sera showed that some proteins were differentially expressed, with most of them showing no differences in composition, shape or density.Being analyzed by MALDI-TOF-MS and database searching,clusterin was differentially expressed and down-regulated in both cancer tissue and pre-surgery serum compared with their counterparts. The results were also validated by RTPCR and western blot.CONCLUSION: The differentially expressed clusterin may play a key role during tumorigenesis of ESCC. The 2DE-MS based proteomic approach is one of the powerful tools for discovery of secreted markers from peripheral.AIM:To identify the differentially secreted proteins or polypeptides associated with tumorigenesis of esophageal squamous cell carcinoma(ESCC)from serum and to find potential tumor secreted biomarkers. METHODS:Proteins from human ESCC tissue and its matched adjacent normal tissue;pre-surgery and post- surgery serum;and pre-surgery and normal control serum were separated by two-dimensional electrophoresis(2-DE) to identify differentially expressed proteins.The silver- stained 2-DE were scanned with digital ImageScanner and analyzed with ImageMaster 2D Elite 3.10 software.A cluster of protein spots differentially expressed were selected and identified with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).One of the differentially expressed proteins,clusterin,was down- regulated in cancer tissue and pre-surgery serum,but it was reversed in post-surgery serum.The results were confirmed by semi-quantitative reverse-transcription(RT)- PCR and western blot. RESULTS:Comparisons of the protein spots identified on the 2-DE maps from human matched sera showed that some proteins were differentially expressed,with most of them showing no differences in composition,shape or density. Being analyzed by MALDI-TOF-MS and database searching, clusterin was differentially expressed and down-regulated in both cancer tissue and pre-surgery serum compared with their counterparts.The results were also validated by RT- PCR and western blot. CONCLUSION:The differentially expressed clusterin may play a key role during tumorigenesis of ESCC.The 2DE-MS based proteomic approach is one of the powerful tools for discovery of secreted markers from peripheral.

关 键 词：食管鳞状细胞癌 肿瘤发生 肿瘤生物学 clusterin基因 基因缺失 蛋白质组学 

分 类 号：R735.1[医药卫生—肿瘤]