转cry1Ac/CpTI双价抗虫水稻Cry1Ac杀虫蛋白的表达特性及其对二化螟的毒杀效果  

Expression of Cry1Ac Protein in cryAc/CpTI Transgenic Riceand Its Resistance in Different Stages to Chilo suppressalis

作　　者：赵红盈[1] 张永军[1] 吴孔明[1] 赵奎军[2] 彭于发[1] 郭予元[1] 

机构地区：[1]中国农业科学院植物保护研究所,北京100094 [2]东北农业大学植保学院,哈尔滨150030

出　　处：《农业生物技术学报》2004年第1期76-79,共4页Journal of Agricultural Biotechnology

基　　金：国家重点基础研究发展规划项目(No.001CB1090);国家高技术研究发展(863)计划(No.2001AA212261)

摘　　要：Cry1Ac杀虫蛋白基因在转cry1Ac/CpTI双价抗虫水稻(OryzasativaL.)各部位中均得到了表达。不同水稻生育期及不同部位中Cry1Ac杀虫蛋白的表达量均有所不同。总的趋势是生育前期的表达量较生育后期高。在时间上,主茎叶片中的杀虫蛋白在拔节期最高,孕穗期开始下降,在扬花期和和灌浆期达到最低,到成熟期略有回升;主茎中的杀虫蛋白在苗期、拔节期和扬花期较高,种子中含量很低。不同生育期转基因水稻主茎顶叶对二化螟(Chilosuppressalis)初孵幼虫表现出了很强的抗虫性。在灌浆期前,取食转基因水稻顶叶的二化螟幼虫校正死亡率均90%左右,灌浆期后抗虫性有所下降,校正死亡率在60%左右。活体成株测定法评价结果表明,转cry1Ac/CpTI双价抗虫水稻对二化螟表现高抗。The expression patterns of Cry1Ac protein and its resistance to stripe d stem borer Chilo suppressalis (Walker) of cry1Ac/CpTI transgenic rice (Oryza sativa L.)were investigated by ELISA and laboratory bioassay. The results from t he ELISA showed that Cry1Ac protein could be detected in all the organs along th e developmental process of cry1Ac/CpTI transgenic rice. The contents of the Cry 1Ac protein varied obviously in different tissues at different stages. Generally , the contents of Cry1Ac protein were higher in earlier stage than that in later stage. The highest levels of the Cry1Ac protein in terminal leaf of main stem w ere found at jointing stage, since then , it decreased at booting stage, and the lowest level was detected at flowering to filling stage, while increased a litt le at maturing stage. Higher levels of Cry1Ac proteins in main stem were found a t seedling stage, jointing stage and flowering stage than that at other stages. There were relative lower level of Cry1Ac proteins in seed. The results from lab oratory bioassay showed that there were high resistance of the terminal leaf of main stem along the developmental process to the newly hatched larvae C. suppres salis. The corrected mortalities of C. suppressalis on the 4th day after feedin g on the detached leaves of cry1Ac/CpTI transgenic rice were up to 90% before f illing stage , while decreased nearly down to 60% after filling stage. It was sh owed that the results of whole plant bioassay were quite coincident with those o f the detached leaf bioassay. The tendency of larval moralities in the detached leaf bioassay and whole plant bioassay were consistent with that of Cry1Ac prot ein contents in different growing periods/organs .

关 键 词：转基因抗虫水稻 杀虫蛋白 表达 二化螟 抗虫性 

分 类 号：S511[农业科学—作物学] S435.112.1