异鼠李素对HeLa细胞端粒酶活性的抑制作用  

Inhibitory Effect of Isorhmnetin on Telomerase Activity of HeLa Cells

作　　者：杨春蕾[1] 屈艺[2] 王正荣[3] 陶大昌[1] 

机构地区：[1]四川大学生命科学院医学细胞生物学教研室,成都610041 [2]四川大学基础医学与法医学院分子生物学与生物化学教研室 [3]四川大学基础医学与法医学院生物医学工程研究室

出　　处：《四川大学学报（医学版）》2004年第2期198-200,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金 (批准号 3 9970 2 75 ;3 0 0 70 2 78)资助

摘　　要：目的　观察异鼠李素对 He L a细胞生长的抑制和诱导凋亡的作用 ,并探讨端粒酶在此过程中的作用。方法　培养细胞加入异鼠李素 ,通过细胞增殖动力学、流式细胞术及 TRAP- EL IAS法检测异鼠李素对 He L a细胞凋亡及端粒酶活性的影响。结果　异鼠李素处理组细胞生长受到抑制 ,抑制作用均与浓度呈梯度关系 ,其 IC50 为(4 0± 0 .0 8) μg/ m l,端粒酶活性较对照组细胞降低 (P<0 .0 5 )。结论　异鼠李素可通过抑制 He LObjective To investigate the growth-inhibiting and apoptosis-inducing effects of isorhmnetin on HeLa cells and to disclose the role of telomerase activity of tumor cells. Methods The methods of cell culture in vitro were adopted. HeLa cells were treated with isorhmnetin in different concentrations for 2 days, and then were observed and analyzed by use of MTT, Flow-Cytometry (FCM) and TRAP-ELIAS technique for inspecting the HeLa cells' growth and telomerase activity. Results The growth of HeLa cells was inhibited evidently after treatment by isorhmnetin. The rate of apoptosis was 31.7% after the HeLa cells were treated with 20 μg/ml isorhmnetin. Isorhmnetin could inhibit the activity of telomerase. Conclusion By inhibiting the activity of telomerase, isorhmnetin can inhibit the growth of HeLa cells.

关 键 词：异鼠李素 凋亡 端粒酶 端粒 永生化 

分 类 号：R73-36[医药卫生—肿瘤]