中国汉族、蒙古族和泰国人群中D8S1132基因座的遗传多态性研究  

Study on the Genetic Polymorphisms of D8S1132 Locus in Chinese Han, Mongolian and Thai Populations

作　　者：周斌[1] 张林[1] 廖淼[1] 贾怡[1] 吕梅励[1] 梁伟波[1] 吴梅筠[1] 

机构地区：[1]四川大学华西基础医学与法医学院法医物证学教研室,成都610041

出　　处：《四川大学学报（医学版）》2004年第2期201-203,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金 (批准号 3 0 1710 3 3 );教育部科研重点项目( 0 114 3 );四川省青年基金资助

摘　　要：目的　获得 D8S1132基因座的群体遗传学数据并分析其基因频率在不同群体间是否有差异。方法　采用PCR、聚丙烯酰胺凝胶电泳及银染技术分析中国成都地区汉族、海拉尔蒙古族和泰国曼谷地区泰国人群三个群体中D8S1132基因座的遗传多态性 ,获得三个群体 D8S1132基因座的群体遗传学数据。结果　从 380份分别采自成都地区汉族、海拉尔蒙古族和曼谷泰国人三个群体的无血缘关系个体的静脉血 ,共发现 11个等位基因 ,观测到 4 2种基因型。观测杂和度为 85 .0 %～ 89.5 % ,个人识别机率为 94 .1%～ 95 .5 % ,观测到的基因型频率分布符合 Hardy- Weinberg平衡定律。等位基因频率的分布在三个群体间有显著差异。结论　 D8S1132基因座个人识别能力高 ,方法简便、灵敏 ,重复性好 。Objective The aim of the present study was to obtain the population genetic data of short tandem repeat(STR) locus D8S1132 and study the difference of allelic frequency distributions among different populations. Methods A sample of 380 unrelated individuals from 3 populations (Han, Mongolian, Thai) were studied using single PCR followed by polyacrylamide gel electrophoresis and silver staining. Results Eleven alleles and 42 genotypes were observed. The observed heterozygosity and the discrimination power were 85.0%-89.5% and 94.1%-95.5%, respectively. The genotype distributions of D8S1132 locus in 3 populations were in accordance with Hardy-Weinberg equilibrium and the difference of allelic frequency distributions among 3 populations was obvious. Conclusion The methods established are easy-to-perform and sensitive. The discrimination power and exclusion chances of this locus are desirable for forensic analysis.

关 键 词：短串联重复序列 聚合酶链反应 D8S1132 遗传多态性 

分 类 号：R394.5[医药卫生—医学遗传学]