汉滩病毒西安分离株84FLi的S片段全基因序列测定及分析  

Sequencing and analysing of complete S segment of 84 FLi, a Hantaan virus isolated from Xi’an

作　　者：李新红[1] 杨为松[1] 杭长寿[2] 白雪帆[1] 马本江[2] 黄长形[1] 

机构地区：[1]第四军医大学唐都医院解放军感染病诊治中心,陕西西安710038 [2]中国预防医学科学院病毒学研究所,北京100052

出　　处：《医学研究生学报》2004年第3期197-199,203,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助课题 (批准号 :3 0 2 0 0 0 10 )

摘　　要：目的 :　测定我国陕西西安出血热肾病综合征 (HFRS)患者流产胎儿肝分离的病毒 84FLi株的全S片段基因序列 ,了解其分子特征 ,并确定其在汉坦病毒 (HV)种系发生中的位置。　方法 :采用逆转录 聚合酶链式反应(RT PCR) ,扩增出S基因的cDNA ,直接用PCR产物或克隆入 pMD18 T载体中测序。　 结果 :84FLi株的全S基因共由 16 88个核苷酸组成 ,四种核苷酸的比例分别为A 31.39%、C 19.2 5 %、G 2 3.0 4 %和T 2 6 .30 % ,GC含量为4 2 .2 9% ,AT含量为 5 7.71%。最大开放读码框为 37～ 132 6 ,共编码 4 2 9个氨基酸。核苷酸序列同源性分析表明 ,84FLi株与汉滩病毒 (HTN)型同源性高于与其他型HTV的同源性。 84FLi株与中国毒株Chen4和RG9同属于一个进化分支 ,而与HTN型国外毒株距离较远 ,属于不同的进化分支。　结论 :84FLi株属于HTN ,并与国内分离的HTN同源性较高。Objective:To study the complete S segment sequence of 84 FLi, a Hantaan virus isolated in Xi’an from a fetal liver whose mother suffered from hemorrhagic fever with renal syndrome(HFRS),learn about its molecular characters and determine its position in Hantavirus phylogenetic tree. Methods:The cDNA of S segment was amplified by RT-PCR. The purified PCR products were sequenced directly or cloned into pMD18-T vector and then sequenced. Results:The complete S segment of 84 FLi strain was composed of 1 688 nucleotides with a single open reading frame, extending from 37 to 1 326 encoding 429 amino acids. Homology analysis showed that the homologies of 84 FLi S segment nucleotide sequences with HTN isolates especially Chinese isolates were higher than those of other Hanta viruses. 84 FLi strain and Chinses isolates Chen4, RG9 were in the same branch. Conclusion:The results suggest that Strain 84 FLi was one of the Hantaan virus,and highly related to other Chinese Hantaan virus isolates.

关 键 词：汉坦病毒 序列分析 系统发生树 84FLi株 

分 类 号：R373[医药卫生—病原生物学]