胃癌癌前病变中医证候与凋亡相关癌基因mRNA表达的关系  

Relationship Between Syndrome Patterns of Traditional Chinese Medicine and Apoptosis- Related Oncogene mRNA Expressions in Precancerous Lesions of Gastric Cancer

作　　者：胡玲[1] 劳绍贤[1] 唐纯志[2] 周福生[1] 黄志新[1] 

机构地区：[1]广州中医药大学脾胃研究所,广州510405 [2]广州中医药大学针灸推拿学院,广州510405

出　　处：《广州中医药大学学报》2004年第2期81-84,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广州中医药大学科学研究与发展总体规划重点及广东省中医药管理局资助课题(编号:GH0016;300011)

摘　　要：【目的】 探讨胃癌癌前病变(PLGC)中医证候与bcl-2癌基因和p53抑癌基因mRNA表达的关系。【方法】经胃镜及病理证实为PLGC病例共40例,其中中度异型增生24例,重度异型增生9例,不完全性结肠化生7例;兼气滞证10例,兼胃热证12例,兼血瘀证18例。采用原位分子杂交的方法检测胃粘膜活检标本bcl-2癌基因和p53抑癌基因mRNA表达。【结果】本组PLGC中,bcl-2癌基因和p53抑癌基因在转录水平均有过度表达,并随病变的进展而表达逐渐增高。在不同的兼证中,bcl-2癌基因和p53抑癌基因mRNA的表达强度依次为兼气滞证<兼胃热证<兼血瘀证。【结论】bcl-2癌基因和p53抑癌基因在PLGC中有异常表达,其表达与不同兼证有关且可能有一定的证候特异性。[Objective] To study the relationship between syndrome patterns of traditional Chinese medicine and bcl- 2 oncogene, p53 suppression gene mRNA expressions in precancerous lesions of gastric cancer (PIGC). [Methods] Forty PLGC cases were endoscopically and pathologically confinned, including 24 cases of moderate dysplasia of gastric mucosa, 9 cases of severe dysplasia, 7 cases of incomplete colonic intestinal metaplasia. Of the 40 cases, 10 were complicated with Qi stagnation, 12 with stomach-heat, and 18 with blood stasis, mRNA expression of bcl- 2 oncogene and p53 suppression gene were detected in situ by molecular hybridization method. [Results] The mRNA overexpression of bcl- 2 oncogene and p53 suppression gene were found in PLGC, and the expression was gradually increased with the progress of lesions. In the complicated cases, the mRNA expression of bcl- 2 oncogene and p53 suppression gene were the least in the cases with Qi stagnation and less in the cases with stomach- heat than in the cases with blood stasis. [Conclusion] Abnormal mRNA expression of bcl- 2 oncogene and p53 suppression gene were found in PLGC cases and related with different complicated cases, indicating the specificity of different syndrome patterns.

关 键 词：胃癌 癌前病变 中医证候 凋亡 癌基因 mRNA 表达 

分 类 号：R735.2[医药卫生—肿瘤]