沙土鼠全脑适应性再灌注的脑保护机制研究  

Study on mechanism of brain protection after global adapted reperfusion in gerbil

作　　者：董斌[1] 邱永明[1] 罗其中[1] 陆兆丰[1] 徐英辉[1] 陆兆辉[2] 

机构地区：[1]上海第二医科大学附属仁济医院神经外科,上海200001 [2]上海第二医科大学附属新华医院心胸外科,上海200127

出　　处：《中华神经外科疾病研究杂志》2004年第2期128-132,共5页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目 (3990 0 1 53) ;上海市科委基金资助项目 (0 0JC1 4 0 4 6)

摘　　要：目的　动态研究不同灌注时间和灌注量对全脑组织缺血损害的恢复程度和即早基因c fos的表达 ,以探明适应性再灌注的脑保护作用机制。方法　沙土鼠随机分 7组 ,实验组夹闭两侧颈总动脉 ,造成沙土鼠全脑缺血模型 ,夹闭 10min后 ,不同开放时间段 (4min ,8min ,10min ,15min ,30min)开放不同脑血流量 (1/ 4 ,1/ 2 ,一次性全开放 )和单纯血液稀释后分别观察缺血海马CA区c fos蛋白的表达 ,及缺血区大脑半球的改善状况。结果　开放 15min ,1/ 2脑血流量时c fos蛋白表达最高 (P <0 .0 5 ) ,海马CA区缺血损害改善最明显 ,开放 15min ,全脑血流量一次性开放时海马CA区损害最严重。结论　①在适应性灌注流量中 ,脑缺血海马区c fos的表达和神经元凋亡呈反相作用关系 ;②低流量灌注有明显改善脑缺血的作用 ;③夹闭 10min后 ,开放 1/ 2脑血流量 ,持续15min的效果比一次性再灌注开放效果要好。Objective The purpose of the study was to evaluate the role of adapted reperfusion(AR) to the brain and the relationship between AR and immediate early genes(IEGs) expression after global ischemic insults.Methods The gerbil global ischemic models were made by bilateral carotid artery 10 mins occlusion. After that, the study models were randomly divided into seven groups, and each group was subdivided into five groups.The c-fos gene expression of the different reperfusion time(4 min, 8 min,10 min, 15 min, 30 min)and different reperfusion flow(1/4,1/2,1/1),and morphological results of hippocampus CA region were observed. Results c-fos expression was highly localized to the selectively vulnerable CA1 neuronal layer after moderate ischemia(P<0.05). Reperfusion 15mins,1/2 reperfusion flow was the best way to increase c-fos expression and improved ischemic insults.Conclusion ①AR effectively increases c-fos expression of hippocampus CA region, and has antiapoptosis role; ②Low reperfusion flow improves ischemic insults; ③The results indicate that 15 mins,1/2 reperfusion flow is better than other reperfusion ways.

关 键 词：沙土鼠 再灌注损伤 脑保护 全脑缺血 C-FOS基因 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R-33[医药卫生—临床医学]