心肌缺血预处理对未成熟心肌细胞凋亡的影响  

The Effects of Myocardial Ischemic Preconditioning on Immature Myocardial Cell Apoptosis

作　　者：孙忠东[1] 池一凡[1] 杨铁南[1] 侯文明[1] 牛兆倬[1] 唐子龙[1] 

机构地区：[1]青岛大学医学院附属青岛市立医院心外科,山东青岛266011

出　　处：《中国体外循环杂志》2004年第1期25-27,43,共4页Chinese Journal of Extracorporeal Circulation

基　　金：国家自然科学基金 (30 2 0 0 2 79)

摘　　要：目的　探讨心肌缺血预处理 (MIP)对未成熟心肌bcl- 2、bax、fas蛋白表达的影响。方法　采用兔Langen dorff模型 ,2 4只幼兔随机分为 3组 ,对照组 (C ,n =8) :仅灌注KH液 180min ,然后取标本 ;缺血 /再灌注组 (I/R ,n=8) :灌注 2 0min后 ,停灌 4 5min ,复灌 12 0min ;心肌缺血预处理组 (MIP ,n =8) ,灌注 2 0min ,反复 2次停灌 5min ,复灌 5min ,然后重复I/R组缺血 /再灌方法。以凋亡细胞原位标记、琼脂糖凝胶电泳检测DNA片段梯及bcl- 2、bax、fas蛋白表达作为检查指标。结果　琼脂糖凝胶电泳检测DNA片段梯 :MIP组与I/R组比较 ,光密度明显减弱 ;MIP组与I/R组比较 ,bcl- 2表达明显增多 ,bax、fas表达明显减少。结论　MIP通过影响bcl- 2、bax、fas基因蛋白的表达减少心肌细胞凋亡。OBJECTIVE To investigate the effects of myocardial ischemic preconditioning (MIP) on immature myocardial cell apoptosis and expression of bcl-2,bax,fas protein .METHODS Isolated working rabbit heart model was used in this study, 24 rabbits (aged 14～21 days) were divided into 3 groups (n=8) . Control group was only perfused with Krebs-Henseleit (KH) 180 minutes. Ischemia/reperfusion(I/R) group was perfused 20 minutes ,and then kept under ischemia 45 minutes, followed by reperfustion 120 minutes. Myocardial ischemic preconditioning (MIP) group was perfused 20 minutes ,and then repeatly ischemia 5 minutes followed reperfusion 5 minutes 2 times,finnaly reperfused 120 minutes. Such indexes, including the TUNEL, nucleosomal ladder of DNA fragments, and the expression of bcl-2, bax,and fas gene were detected.RESULTS MIP group there was reduction in TUNEL cell apoptosis, ladder of DNA fragments,expression of bax and fas gene,and increase in expression of bcl-2 gene.CONCLUSION This study demonstrated that MIP decrease immature myocardial cell apoptosis by affecting the expression of bcl-2, bax,and fas gene .

关 键 词：心肌缺血 未成熟心肌 缺血预处理 细胞凋亡 心肌细胞 再灌注损伤 

分 类 号：R363[医药卫生—病理学]