口腔癌前病变、口腔鳞癌中诱导型一氧化氮合酶与p53蛋白表达及细胞增殖相互关系的研究  

Correlation between inducible nitric oxide synthase and p53 protein expression, cellular proliferation in the trans-formation of oral squamous cell carcinoma

作　　者：杨萍[1] 高文信[2] 魏秀峰[2] 王雪[2] 莫珩[2] 

机构地区：[1]哈尔滨工业大学口腔医学中心 [2]吉林大学口腔医院,吉林长春130041

出　　处：《临床口腔医学杂志》2004年第3期143-145,共3页Journal of Clinical Stomatology

摘　　要：目的:探讨诱导型一氧化氮合酶(inducible netric oxide synthase,iNOS)在口腔鳞癌发展过程中的表达及与p53蛋白表达和细胞增殖的相互关系。方法:采用免疫组化SABC法检测10例正常口腔黏膜、8例上皮单纯增生、20例上皮异常增生和32例口腔鳞癌组织中iNOS、p53及Ki-67蛋白的表达。结果:口腔鳞癌发展过程中存在着iNOS、p53、Ki-67表达的上调;三种蛋白的表达与上皮异常增生的病理分级有关(P<0.05),与鳞癌的病理分级无关;各组中iNOS和p53表达之间均存在显著正相关(P<0.05);iNOS阳性表达的鳞癌组织中Ki-67标记指数较阴性者显著增高(P<0.05)。结论:iNOS与p53蛋白互为反馈影响,在口腔鳞癌发展过程中意义重大;鳞癌中iNOS的阳性表达可能提示该组织的快速增殖优势。Objective:To study the expression of iNOS and p53 staining, cellular proliferation in the oral squamous cell carcinoma(OSCC). Method: SABC immunohistochemical technique was used to detect the iNOS, p53 and Ki - 67 expression in; 10 cases of nomal oral rnucosa, 8 cases of oral epithelial hyperplasia, 20 cases of oral epithelial dysplasia and 32 cases of OSCC. Result:Staining for iNOS, p53 or Ki - 67 was upgradulated in the transformation of OSCC. Three indices independently correlated with grade of dysplasia(P<0.05) . A positive correlation was found between iNOS and p53 expression in all groups(P< . 005) . Statistical analysis also showed the cell proliferation index of iNOS - positive tumors was significantly higher than iNOS - negative tumors(P<0.05). Conclusion: The correlation between iNOS and p53 may play important roles in OSCC development. In OSCC, the positive staining of iNOS may confer a proliferative adventage to these tumors.

关 键 词：口腔癌 癌前病变 口腔鳞癌 一氧化氮合酶 P53蛋白表达 细胞增殖 INOS 肿瘤 

分 类 号：R739.8[医药卫生—肿瘤]